Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_12 and Pf6N2E2_13 are separated by 24 nucleotides Pf6N2E2_13 and Pf6N2E2_14 overlap by 4 nucleotides
Pf6N2E2_12 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 41,033 to 41,908
_12
Pf6N2E2_13 - Pyoverdin biosynthesis protein PvdN, putative aminotransferase, class V, at 41,933 to 43,213
_13
Pf6N2E2_14 - Putative dipeptidase, pyoverdin biosynthesis PvdM, at 43,210 to 44,580
_14
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 41.034 kb on + strand at 41.034 kb on + strand at 41.034 kb on + strand at 41.034 kb on + strand at 41.034 kb on + strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.035 kb on - strand at 41.038 kb on + strand at 41.208 kb on + strand, within Pf6N2E2_12 at 41.209 kb on - strand, within Pf6N2E2_12 at 41.209 kb on - strand, within Pf6N2E2_12 at 41.221 kb on - strand, within Pf6N2E2_12 at 41.221 kb on - strand, within Pf6N2E2_12 at 41.241 kb on + strand, within Pf6N2E2_12 at 41.262 kb on + strand, within Pf6N2E2_12 at 41.331 kb on + strand, within Pf6N2E2_12 at 41.359 kb on - strand, within Pf6N2E2_12 at 41.484 kb on + strand, within Pf6N2E2_12 at 41.484 kb on + strand, within Pf6N2E2_12 at 41.484 kb on + strand, within Pf6N2E2_12 at 41.485 kb on - strand, within Pf6N2E2_12 at 41.535 kb on + strand, within Pf6N2E2_12 at 41.535 kb on + strand, within Pf6N2E2_12 at 41.535 kb on + strand, within Pf6N2E2_12 at 41.535 kb on + strand, within Pf6N2E2_12 at 41.535 kb on + strand, within Pf6N2E2_12 at 41.536 kb on - strand, within Pf6N2E2_12 at 41.536 kb on - strand, within Pf6N2E2_12 at 41.536 kb on - strand, within Pf6N2E2_12 at 41.623 kb on - strand, within Pf6N2E2_12 at 42.189 kb on + strand, within Pf6N2E2_13 at 42.360 kb on + strand, within Pf6N2E2_13 at 42.361 kb on - strand, within Pf6N2E2_13 at 42.361 kb on - strand, within Pf6N2E2_13 at 42.507 kb on + strand, within Pf6N2E2_13 at 42.508 kb on - strand, within Pf6N2E2_13 at 42.508 kb on - strand, within Pf6N2E2_13 at 42.508 kb on - strand, within Pf6N2E2_13 at 42.508 kb on - strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.823 kb on - strand, within Pf6N2E2_13 at 42.823 kb on - strand, within Pf6N2E2_13 at 42.971 kb on - strand, within Pf6N2E2_13 at 43.096 kb on + strand at 43.096 kb on + strand at 43.097 kb on - strand at 43.387 kb on + strand, within Pf6N2E2_14 at 43.387 kb on + strand, within Pf6N2E2_14 at 43.387 kb on + strand, within Pf6N2E2_14 at 43.388 kb on - strand, within Pf6N2E2_14 at 43.388 kb on - strand, within Pf6N2E2_14 at 43.455 kb on - strand, within Pf6N2E2_14 at 43.676 kb on + strand, within Pf6N2E2_14 at 43.803 kb on + strand, within Pf6N2E2_14 at 43.991 kb on + strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 44.135 kb on + strand, within Pf6N2E2_14 at 44.135 kb on + strand, within Pf6N2E2_14
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 41,034 + -0.1 41,034 + +4.1 41,034 + -0.5 41,034 + -2.7 41,034 + +1.5 41,035 - +0.4 41,035 - -0.9 41,035 - +0.6 41,035 - -0.6 41,035 - -4.5 41,035 - +0.2 41,035 - -0.5 41,035 - -0.1 41,035 - -0.6 41,035 - -0.6 41,035 - -0.1 41,038 + -2.8 41,208 + Pf6N2E2_12 0.20 +1.1 41,209 - Pf6N2E2_12 0.20 -0.1 41,209 - Pf6N2E2_12 0.20 +1.2 41,221 - Pf6N2E2_12 0.21 +0.7 41,221 - Pf6N2E2_12 0.21 -2.9 41,241 + Pf6N2E2_12 0.24 -2.7 41,262 + Pf6N2E2_12 0.26 +1.7 41,331 + Pf6N2E2_12 0.34 +1.1 41,359 - Pf6N2E2_12 0.37 +2.5 41,484 + Pf6N2E2_12 0.51 +1.6 41,484 + Pf6N2E2_12 0.51 -0.3 41,484 + Pf6N2E2_12 0.51 -2.1 41,485 - Pf6N2E2_12 0.52 -1.7 41,535 + Pf6N2E2_12 0.57 +1.5 41,535 + Pf6N2E2_12 0.57 -1.0 41,535 + Pf6N2E2_12 0.57 -1.8 41,535 + Pf6N2E2_12 0.57 +0.6 41,535 + Pf6N2E2_12 0.57 -1.4 41,536 - Pf6N2E2_12 0.57 -2.5 41,536 - Pf6N2E2_12 0.57 +1.3 41,536 - Pf6N2E2_12 0.57 -1.7 41,623 - Pf6N2E2_12 0.67 +0.4 42,189 + Pf6N2E2_13 0.20 -1.1 42,360 + Pf6N2E2_13 0.33 +1.3 42,361 - Pf6N2E2_13 0.33 +3.1 42,361 - Pf6N2E2_13 0.33 +1.6 42,507 + Pf6N2E2_13 0.45 +0.8 42,508 - Pf6N2E2_13 0.45 +1.9 42,508 - Pf6N2E2_13 0.45 -0.4 42,508 - Pf6N2E2_13 0.45 +0.8 42,508 - Pf6N2E2_13 0.45 +0.8 42,822 + Pf6N2E2_13 0.69 +1.0 42,822 + Pf6N2E2_13 0.69 +0.4 42,822 + Pf6N2E2_13 0.69 -2.4 42,822 + Pf6N2E2_13 0.69 -0.1 42,822 + Pf6N2E2_13 0.69 +1.6 42,823 - Pf6N2E2_13 0.69 -0.2 42,823 - Pf6N2E2_13 0.69 +1.5 42,971 - Pf6N2E2_13 0.81 +0.5 43,096 + +0.9 43,096 + -2.9 43,097 - +0.8 43,387 + Pf6N2E2_14 0.13 +0.6 43,387 + Pf6N2E2_14 0.13 -1.2 43,387 + Pf6N2E2_14 0.13 -0.8 43,388 - Pf6N2E2_14 0.13 -0.1 43,388 - Pf6N2E2_14 0.13 -2.0 43,455 - Pf6N2E2_14 0.18 +1.1 43,676 + Pf6N2E2_14 0.34 +1.6 43,803 + Pf6N2E2_14 0.43 -0.3 43,991 + Pf6N2E2_14 0.57 +0.1 43,992 - Pf6N2E2_14 0.57 -1.0 43,992 - Pf6N2E2_14 0.57 -0.5 43,992 - Pf6N2E2_14 0.57 +0.7 44,135 + Pf6N2E2_14 0.67 -0.5 44,135 + Pf6N2E2_14 0.67 -2.2
Or see this region's nucleotide sequence