Experiment: Hydroxylamine 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1166 and Pf6N2E2_1167 are separated by 77 nucleotides Pf6N2E2_1167 and Pf6N2E2_1168 are separated by 61 nucleotides Pf6N2E2_1168 and Pf6N2E2_1169 are separated by 27 nucleotides
Pf6N2E2_1166 - Mobile element protein, at 1,367,174 to 1,367,464
_1166
Pf6N2E2_1167 - Transposase, at 1,367,542 to 1,367,838
_1167
Pf6N2E2_1168 - Transposase, at 1,367,900 to 1,368,298
_1168
Pf6N2E2_1169 - TnpR protein, at 1,368,326 to 1,368,886
_1169
Position (kb)
1367
1368
1369 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1366.937 kb on + strand at 1366.941 kb on + strand at 1366.964 kb on + strand at 1367.011 kb on + strand at 1367.021 kb on + strand at 1367.133 kb on + strand at 1367.311 kb on - strand, within Pf6N2E2_1166 at 1367.489 kb on - strand at 1367.520 kb on + strand at 1367.520 kb on + strand at 1367.521 kb on - strand at 1367.979 kb on - strand, within Pf6N2E2_1168 at 1368.093 kb on - strand, within Pf6N2E2_1168 at 1368.141 kb on - strand, within Pf6N2E2_1168 at 1368.142 kb on + strand, within Pf6N2E2_1168 at 1368.188 kb on + strand, within Pf6N2E2_1168 at 1368.189 kb on - strand, within Pf6N2E2_1168 at 1368.189 kb on - strand, within Pf6N2E2_1168 at 1368.189 kb on - strand, within Pf6N2E2_1168 at 1368.368 kb on - strand at 1368.578 kb on + strand, within Pf6N2E2_1169 at 1368.578 kb on + strand, within Pf6N2E2_1169 at 1368.579 kb on - strand, within Pf6N2E2_1169 at 1368.599 kb on + strand, within Pf6N2E2_1169 at 1368.982 kb on + strand at 1369.230 kb on + strand at 1369.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxylamine 0.5 M remove 1,366,937 + -2.3 1,366,941 + +0.8 1,366,964 + +0.9 1,367,011 + -0.1 1,367,021 + +2.2 1,367,133 + +1.2 1,367,311 - Pf6N2E2_1166 0.47 +0.6 1,367,489 - -2.1 1,367,520 + -0.0 1,367,520 + -1.4 1,367,521 - -0.2 1,367,979 - Pf6N2E2_1168 0.20 -0.8 1,368,093 - Pf6N2E2_1168 0.48 -0.6 1,368,141 - Pf6N2E2_1168 0.60 -1.0 1,368,142 + Pf6N2E2_1168 0.61 -1.6 1,368,188 + Pf6N2E2_1168 0.72 +0.2 1,368,189 - Pf6N2E2_1168 0.72 -1.8 1,368,189 - Pf6N2E2_1168 0.72 +0.6 1,368,189 - Pf6N2E2_1168 0.72 -3.0 1,368,368 - -0.8 1,368,578 + Pf6N2E2_1169 0.45 +0.6 1,368,578 + Pf6N2E2_1169 0.45 -2.7 1,368,579 - Pf6N2E2_1169 0.45 +1.0 1,368,599 + Pf6N2E2_1169 0.49 -2.3 1,368,982 + -1.4 1,369,230 + -0.4 1,369,295 + +0.6
Or see this region's nucleotide sequence