Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25320

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25315 and BWI76_RS25320 are separated by 9 nucleotidesBWI76_RS25320 and BWI76_RS25325 are separated by 79 nucleotidesBWI76_RS25325 and BWI76_RS25330 are separated by 92 nucleotides BWI76_RS25315: BWI76_RS25315 - glutamate synthase large subunit, at 5,261,068 to 5,265,528 _RS25315 BWI76_RS25320: BWI76_RS25320 - glutamate synthase, at 5,265,538 to 5,266,956 _RS25320 BWI76_RS25325: BWI76_RS25325 - hypothetical protein, at 5,267,036 to 5,267,443 _RS25325 BWI76_RS25330: BWI76_RS25330 - stringent starvation protein B, at 5,267,536 to 5,268,024 _RS25330 Position (kb) 5265 5266 5267Strain fitness (log2 ratio) -3 -2 -1 0 1at 5264.659 kb on + strand, within BWI76_RS25315at 5264.672 kb on - strand, within BWI76_RS25315at 5264.763 kb on - strand, within BWI76_RS25315at 5264.768 kb on + strand, within BWI76_RS25315at 5264.919 kb on - strand, within BWI76_RS25315at 5265.056 kb on - strand, within BWI76_RS25315at 5265.502 kb on + strandat 5265.748 kb on - strand, within BWI76_RS25320at 5265.967 kb on - strand, within BWI76_RS25320at 5266.009 kb on + strand, within BWI76_RS25320at 5266.228 kb on + strand, within BWI76_RS25320at 5266.268 kb on - strand, within BWI76_RS25320at 5266.308 kb on + strand, within BWI76_RS25320at 5266.432 kb on + strand, within BWI76_RS25320at 5266.432 kb on + strand, within BWI76_RS25320at 5266.561 kb on - strand, within BWI76_RS25320at 5266.611 kb on + strand, within BWI76_RS25320at 5266.691 kb on + strand, within BWI76_RS25320at 5267.041 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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5,264,659 + BWI76_RS25315 0.80 -1.1
5,264,672 - BWI76_RS25315 0.81 -1.7
5,264,763 - BWI76_RS25315 0.83 -0.6
5,264,768 + BWI76_RS25315 0.83 -0.4
5,264,919 - BWI76_RS25315 0.86 -2.8
5,265,056 - BWI76_RS25315 0.89 -1.2
5,265,502 + +1.6
5,265,748 - BWI76_RS25320 0.15 -1.6
5,265,967 - BWI76_RS25320 0.30 -2.2
5,266,009 + BWI76_RS25320 0.33 -1.9
5,266,228 + BWI76_RS25320 0.49 -1.4
5,266,268 - BWI76_RS25320 0.51 +0.4
5,266,308 + BWI76_RS25320 0.54 -0.6
5,266,432 + BWI76_RS25320 0.63 -0.5
5,266,432 + BWI76_RS25320 0.63 -0.2
5,266,561 - BWI76_RS25320 0.72 -1.5
5,266,611 + BWI76_RS25320 0.76 -1.7
5,266,691 + BWI76_RS25320 0.81 -1.4
5,267,041 - +0.7

Or see this region's nucleotide sequence