Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17050

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17040 and BWI76_RS17045 are separated by 336 nucleotidesBWI76_RS17045 and BWI76_RS17050 overlap by 4 nucleotidesBWI76_RS17050 and BWI76_RS17055 are separated by 203 nucleotides BWI76_RS17040: BWI76_RS17040 - GlsB/YeaQ/YmgE family stress response membrane protein, at 3,534,286 to 3,534,534 _RS17040 BWI76_RS17045: BWI76_RS17045 - hypothetical protein, at 3,534,871 to 3,535,281 _RS17045 BWI76_RS17050: BWI76_RS17050 - FAD-binding oxidoreductase, at 3,535,278 to 3,538,334 _RS17050 BWI76_RS17055: BWI76_RS17055 - hypothetical protein, at 3,538,538 to 3,539,659 _RS17055 Position (kb) 3535 3536 3537 3538 3539Strain fitness (log2 ratio) -1 0 1 2at 3534.322 kb on - strand, within BWI76_RS17040at 3534.334 kb on - strand, within BWI76_RS17040at 3534.342 kb on + strand, within BWI76_RS17040at 3534.392 kb on + strand, within BWI76_RS17040at 3534.392 kb on + strand, within BWI76_RS17040at 3534.400 kb on - strand, within BWI76_RS17040at 3534.400 kb on - strand, within BWI76_RS17040at 3534.400 kb on - strand, within BWI76_RS17040at 3534.414 kb on - strand, within BWI76_RS17040at 3534.414 kb on - strand, within BWI76_RS17040at 3534.424 kb on - strand, within BWI76_RS17040at 3534.471 kb on + strand, within BWI76_RS17040at 3534.654 kb on + strandat 3534.654 kb on + strandat 3534.654 kb on + strandat 3534.654 kb on + strandat 3534.662 kb on - strandat 3534.708 kb on - strandat 3534.788 kb on - strandat 3534.823 kb on + strandat 3534.823 kb on + strandat 3534.867 kb on - strandat 3534.877 kb on - strandat 3534.877 kb on - strandat 3535.176 kb on - strand, within BWI76_RS17045at 3535.241 kb on + strandat 3535.574 kb on + strandat 3535.614 kb on + strand, within BWI76_RS17050at 3536.149 kb on - strand, within BWI76_RS17050at 3536.596 kb on + strand, within BWI76_RS17050at 3536.612 kb on - strand, within BWI76_RS17050at 3536.623 kb on + strand, within BWI76_RS17050at 3536.729 kb on + strand, within BWI76_RS17050at 3536.996 kb on - strand, within BWI76_RS17050at 3537.001 kb on - strand, within BWI76_RS17050at 3537.051 kb on - strand, within BWI76_RS17050at 3537.243 kb on - strand, within BWI76_RS17050at 3537.454 kb on - strand, within BWI76_RS17050at 3537.499 kb on + strand, within BWI76_RS17050at 3537.675 kb on - strand, within BWI76_RS17050at 3537.685 kb on + strand, within BWI76_RS17050at 3537.858 kb on - strand, within BWI76_RS17050at 3537.880 kb on + strand, within BWI76_RS17050at 3538.096 kb on - strandat 3538.096 kb on - strandat 3538.214 kb on + strandat 3538.214 kb on + strandat 3538.372 kb on + strandat 3538.487 kb on + strandat 3538.524 kb on + strandat 3538.574 kb on + strandat 3538.643 kb on - strandat 3538.665 kb on - strand, within BWI76_RS17055at 3538.813 kb on - strand, within BWI76_RS17055at 3538.896 kb on + strand, within BWI76_RS17055at 3538.903 kb on + strand, within BWI76_RS17055at 3538.903 kb on + strand, within BWI76_RS17055at 3538.911 kb on - strand, within BWI76_RS17055at 3539.053 kb on + strand, within BWI76_RS17055at 3539.214 kb on - strand, within BWI76_RS17055at 3539.214 kb on - strand, within BWI76_RS17055at 3539.232 kb on + strand, within BWI76_RS17055at 3539.240 kb on - strand, within BWI76_RS17055at 3539.325 kb on - strand, within BWI76_RS17055

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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3,534,322 - BWI76_RS17040 0.14 +1.8
3,534,334 - BWI76_RS17040 0.19 -0.3
3,534,342 + BWI76_RS17040 0.22 -1.6
3,534,392 + BWI76_RS17040 0.43 -0.3
3,534,392 + BWI76_RS17040 0.43 -0.1
3,534,400 - BWI76_RS17040 0.46 +0.7
3,534,400 - BWI76_RS17040 0.46 +0.1
3,534,400 - BWI76_RS17040 0.46 +0.1
3,534,414 - BWI76_RS17040 0.51 +0.7
3,534,414 - BWI76_RS17040 0.51 +0.2
3,534,424 - BWI76_RS17040 0.55 -0.3
3,534,471 + BWI76_RS17040 0.74 +0.1
3,534,654 + +0.2
3,534,654 + -0.1
3,534,654 + +0.4
3,534,654 + -0.3
3,534,662 - -0.1
3,534,708 - +0.1
3,534,788 - -0.7
3,534,823 + +0.3
3,534,823 + -0.1
3,534,867 - -0.0
3,534,877 - -0.6
3,534,877 - -0.2
3,535,176 - BWI76_RS17045 0.74 +1.1
3,535,241 + -0.2
3,535,574 + +0.5
3,535,614 + BWI76_RS17050 0.11 -0.5
3,536,149 - BWI76_RS17050 0.28 -0.7
3,536,596 + BWI76_RS17050 0.43 -0.3
3,536,612 - BWI76_RS17050 0.44 -0.4
3,536,623 + BWI76_RS17050 0.44 -1.4
3,536,729 + BWI76_RS17050 0.47 +1.4
3,536,996 - BWI76_RS17050 0.56 +0.0
3,537,001 - BWI76_RS17050 0.56 -0.1
3,537,051 - BWI76_RS17050 0.58 +1.0
3,537,243 - BWI76_RS17050 0.64 -0.7
3,537,454 - BWI76_RS17050 0.71 +1.2
3,537,499 + BWI76_RS17050 0.73 -0.1
3,537,675 - BWI76_RS17050 0.78 -0.1
3,537,685 + BWI76_RS17050 0.79 +0.2
3,537,858 - BWI76_RS17050 0.84 -0.8
3,537,880 + BWI76_RS17050 0.85 +0.3
3,538,096 - -0.3
3,538,096 - +0.7
3,538,214 + +0.2
3,538,214 + -0.9
3,538,372 + -0.2
3,538,487 + -1.1
3,538,524 + +0.2
3,538,574 + +0.2
3,538,643 - +0.4
3,538,665 - BWI76_RS17055 0.11 +0.1
3,538,813 - BWI76_RS17055 0.25 +0.8
3,538,896 + BWI76_RS17055 0.32 -0.9
3,538,903 + BWI76_RS17055 0.33 -0.1
3,538,903 + BWI76_RS17055 0.33 +0.8
3,538,911 - BWI76_RS17055 0.33 -0.5
3,539,053 + BWI76_RS17055 0.46 -0.0
3,539,214 - BWI76_RS17055 0.60 -0.6
3,539,214 - BWI76_RS17055 0.60 +0.4
3,539,232 + BWI76_RS17055 0.62 +0.6
3,539,240 - BWI76_RS17055 0.63 -0.1
3,539,325 - BWI76_RS17055 0.70 -0.6

Or see this region's nucleotide sequence