Experiment: Recovery broth with Kanamycin sulfate 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13835 and BBR_RS13840 are separated by 123 nucleotides BBR_RS13840 and BBR_RS13845 are separated by 91 nucleotides
BBR_RS13835: BBR_RS13835 - FAD-dependent oxidoreductase, at 882,198 to 883,691
_RS13835
BBR_RS13840: BBR_RS13840 - DUF3043 domain-containing protein, at 883,815 to 884,435
_RS13840
BBR_RS13845: BBR_RS13845 - dipeptidase, at 884,527 to 885,894
_RS13845
Position (kb) 883
884
885 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 882.840 kb on + strand, within BBR_RS13835 at 882.840 kb on + strand, within BBR_RS13835 at 882.848 kb on - strand, within BBR_RS13835 at 882.848 kb on - strand, within BBR_RS13835 at 882.868 kb on + strand, within BBR_RS13835 at 882.899 kb on - strand, within BBR_RS13835 at 882.899 kb on - strand, within BBR_RS13835 at 882.951 kb on + strand, within BBR_RS13835 at 882.985 kb on - strand, within BBR_RS13835 at 883.014 kb on + strand, within BBR_RS13835 at 883.088 kb on - strand, within BBR_RS13835 at 883.171 kb on + strand, within BBR_RS13835 at 883.309 kb on + strand, within BBR_RS13835 at 883.364 kb on + strand, within BBR_RS13835 at 883.369 kb on + strand, within BBR_RS13835 at 883.399 kb on + strand, within BBR_RS13835 at 883.427 kb on + strand, within BBR_RS13835 at 883.454 kb on - strand, within BBR_RS13835 at 883.535 kb on - strand, within BBR_RS13835 at 883.557 kb on + strand at 883.565 kb on - strand at 883.565 kb on - strand at 883.565 kb on - strand at 883.566 kb on + strand at 883.607 kb on - strand at 883.622 kb on - strand at 883.722 kb on + strand at 883.723 kb on + strand at 883.738 kb on - strand at 883.797 kb on + strand at 883.843 kb on + strand at 883.872 kb on - strand at 883.926 kb on - strand, within BBR_RS13840 at 883.959 kb on + strand, within BBR_RS13840 at 883.968 kb on - strand, within BBR_RS13840 at 883.968 kb on - strand, within BBR_RS13840 at 884.053 kb on - strand, within BBR_RS13840 at 884.055 kb on - strand, within BBR_RS13840 at 884.190 kb on - strand, within BBR_RS13840 at 884.200 kb on + strand, within BBR_RS13840 at 884.322 kb on - strand, within BBR_RS13840 at 884.423 kb on + strand at 884.431 kb on - strand at 884.475 kb on + strand at 884.475 kb on + strand at 884.475 kb on + strand at 884.509 kb on + strand at 884.550 kb on - strand at 884.758 kb on + strand, within BBR_RS13845 at 884.797 kb on + strand, within BBR_RS13845 at 884.802 kb on + strand, within BBR_RS13845 at 884.814 kb on + strand, within BBR_RS13845 at 884.815 kb on - strand, within BBR_RS13845 at 884.821 kb on + strand, within BBR_RS13845 at 884.898 kb on - strand, within BBR_RS13845 at 884.966 kb on + strand, within BBR_RS13845 at 884.987 kb on - strand, within BBR_RS13845 at 885.004 kb on + strand, within BBR_RS13845 at 885.031 kb on - strand, within BBR_RS13845 at 885.089 kb on - strand, within BBR_RS13845 at 885.109 kb on + strand, within BBR_RS13845 at 885.114 kb on - strand, within BBR_RS13845 at 885.128 kb on - strand, within BBR_RS13845 at 885.136 kb on - strand, within BBR_RS13845 at 885.146 kb on + strand, within BBR_RS13845 at 885.180 kb on + strand, within BBR_RS13845 at 885.193 kb on - strand, within BBR_RS13845 at 885.218 kb on + strand, within BBR_RS13845 at 885.237 kb on + strand, within BBR_RS13845 at 885.420 kb on + strand, within BBR_RS13845
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Kanamycin sulfate 0.8 mM remove 882,840 + BBR_RS13835 0.43 +0.4 882,840 + BBR_RS13835 0.43 -0.0 882,848 - BBR_RS13835 0.44 +0.8 882,848 - BBR_RS13835 0.44 +0.1 882,868 + BBR_RS13835 0.45 +0.5 882,899 - BBR_RS13835 0.47 +0.2 882,899 - BBR_RS13835 0.47 -0.3 882,951 + BBR_RS13835 0.50 +1.2 882,985 - BBR_RS13835 0.53 +0.1 883,014 + BBR_RS13835 0.55 +1.0 883,088 - BBR_RS13835 0.60 +0.7 883,171 + BBR_RS13835 0.65 -0.3 883,309 + BBR_RS13835 0.74 -0.2 883,364 + BBR_RS13835 0.78 +0.7 883,369 + BBR_RS13835 0.78 +0.8 883,399 + BBR_RS13835 0.80 -0.6 883,427 + BBR_RS13835 0.82 +0.7 883,454 - BBR_RS13835 0.84 -2.0 883,535 - BBR_RS13835 0.89 -0.0 883,557 + -0.4 883,565 - +1.1 883,565 - -0.5 883,565 - -0.2 883,566 + -1.6 883,607 - +0.4 883,622 - -1.1 883,722 + -0.3 883,723 + +0.1 883,738 - +0.0 883,797 + -0.4 883,843 + -1.5 883,872 - -2.6 883,926 - BBR_RS13840 0.18 -4.1 883,959 + BBR_RS13840 0.23 -2.6 883,968 - BBR_RS13840 0.25 -1.5 883,968 - BBR_RS13840 0.25 -2.3 884,053 - BBR_RS13840 0.38 -2.1 884,055 - BBR_RS13840 0.39 -2.6 884,190 - BBR_RS13840 0.60 -0.9 884,200 + BBR_RS13840 0.62 -3.1 884,322 - BBR_RS13840 0.82 -0.9 884,423 + -2.8 884,431 - -2.0 884,475 + +1.4 884,475 + +0.6 884,475 + +0.3 884,509 + +0.7 884,550 - +0.2 884,758 + BBR_RS13845 0.17 -0.2 884,797 + BBR_RS13845 0.20 +0.1 884,802 + BBR_RS13845 0.20 +0.2 884,814 + BBR_RS13845 0.21 -2.0 884,815 - BBR_RS13845 0.21 +0.4 884,821 + BBR_RS13845 0.21 -0.2 884,898 - BBR_RS13845 0.27 -0.2 884,966 + BBR_RS13845 0.32 +0.1 884,987 - BBR_RS13845 0.34 +0.3 885,004 + BBR_RS13845 0.35 -0.1 885,031 - BBR_RS13845 0.37 +0.5 885,089 - BBR_RS13845 0.41 -0.3 885,109 + BBR_RS13845 0.43 -0.9 885,114 - BBR_RS13845 0.43 -0.1 885,128 - BBR_RS13845 0.44 +1.6 885,136 - BBR_RS13845 0.45 +0.2 885,146 + BBR_RS13845 0.45 -1.0 885,180 + BBR_RS13845 0.48 +0.9 885,193 - BBR_RS13845 0.49 -0.2 885,218 + BBR_RS13845 0.51 -1.6 885,237 + BBR_RS13845 0.52 -1.8 885,420 + BBR_RS13845 0.65 -0.0
Or see this region's nucleotide sequence