Experiment: Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_30355 and AO356_30360 are separated by 63 nucleotides AO356_30360 and AO356_30365 are separated by 49 nucleotides AO356_30365 and AO356_30370 are separated by 68 nucleotides
AO356_30355: AO356_30355 - enoyl-CoA hydratase, at 7,085,525 to 7,086,319
_30355
AO356_30360: AO356_30360 - 3-hydroxypropionyl-CoA dehydratase, at 7,086,383 to 7,087,120
_30360
AO356_30365: AO356_30365 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 7,087,170 to 7,088,210
_30365
AO356_30370: AO356_30370 - CoA-binding protein, at 7,088,279 to 7,090,393
_30370
Position (kb) 7087
7088
7089 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 7086.278 kb on + strand at 7086.279 kb on - strand at 7086.299 kb on - strand at 7086.322 kb on + strand at 7086.323 kb on - strand at 7086.323 kb on - strand at 7086.560 kb on + strand, within AO356_30360 at 7086.659 kb on + strand, within AO356_30360 at 7086.659 kb on + strand, within AO356_30360 at 7086.881 kb on - strand, within AO356_30360 at 7087.003 kb on + strand, within AO356_30360 at 7087.004 kb on - strand, within AO356_30360 at 7087.004 kb on - strand, within AO356_30360 at 7087.015 kb on + strand, within AO356_30360 at 7087.016 kb on - strand, within AO356_30360 at 7087.104 kb on - strand at 7087.104 kb on - strand at 7087.129 kb on - strand at 7087.129 kb on - strand at 7087.143 kb on - strand at 7087.172 kb on - strand at 7087.172 kb on - strand at 7087.292 kb on - strand, within AO356_30365 at 7087.365 kb on + strand, within AO356_30365 at 7087.366 kb on - strand, within AO356_30365 at 7087.366 kb on - strand, within AO356_30365 at 7087.398 kb on + strand, within AO356_30365 at 7087.399 kb on - strand, within AO356_30365 at 7087.399 kb on - strand, within AO356_30365 at 7087.399 kb on - strand, within AO356_30365 at 7087.451 kb on - strand, within AO356_30365 at 7087.481 kb on - strand, within AO356_30365 at 7087.488 kb on + strand, within AO356_30365 at 7087.488 kb on + strand, within AO356_30365 at 7087.488 kb on + strand, within AO356_30365 at 7087.684 kb on + strand, within AO356_30365 at 7087.752 kb on - strand, within AO356_30365 at 7087.777 kb on + strand, within AO356_30365 at 7087.777 kb on + strand, within AO356_30365 at 7087.778 kb on - strand, within AO356_30365 at 7087.778 kb on - strand, within AO356_30365 at 7087.889 kb on + strand, within AO356_30365 at 7087.890 kb on - strand, within AO356_30365 at 7088.121 kb on + strand at 7088.122 kb on - strand at 7088.122 kb on - strand at 7088.122 kb on - strand at 7088.240 kb on + strand at 7088.240 kb on + strand at 7088.240 kb on + strand at 7088.328 kb on - strand at 7088.328 kb on - strand at 7088.360 kb on - strand at 7088.550 kb on + strand, within AO356_30370 at 7088.550 kb on + strand, within AO356_30370 at 7088.570 kb on + strand, within AO356_30370 at 7088.571 kb on - strand, within AO356_30370 at 7088.760 kb on - strand, within AO356_30370 at 7088.805 kb on - strand, within AO356_30370 at 7088.952 kb on - strand, within AO356_30370
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 40 mM remove 7,086,278 + -0.7 7,086,279 - -1.0 7,086,299 - -1.0 7,086,322 + -0.3 7,086,323 - +0.3 7,086,323 - -0.9 7,086,560 + AO356_30360 0.24 -0.7 7,086,659 + AO356_30360 0.37 -0.1 7,086,659 + AO356_30360 0.37 -0.4 7,086,881 - AO356_30360 0.67 -0.1 7,087,003 + AO356_30360 0.84 -0.6 7,087,004 - AO356_30360 0.84 -0.3 7,087,004 - AO356_30360 0.84 -0.7 7,087,015 + AO356_30360 0.86 -0.4 7,087,016 - AO356_30360 0.86 +0.6 7,087,104 - +0.7 7,087,104 - +0.3 7,087,129 - +0.7 7,087,129 - +1.3 7,087,143 - -0.3 7,087,172 - -1.7 7,087,172 - +3.0 7,087,292 - AO356_30365 0.12 -2.3 7,087,365 + AO356_30365 0.19 +0.1 7,087,366 - AO356_30365 0.19 +1.2 7,087,366 - AO356_30365 0.19 -0.6 7,087,398 + AO356_30365 0.22 +0.4 7,087,399 - AO356_30365 0.22 -0.0 7,087,399 - AO356_30365 0.22 +0.1 7,087,399 - AO356_30365 0.22 -0.5 7,087,451 - AO356_30365 0.27 -1.6 7,087,481 - AO356_30365 0.30 -0.1 7,087,488 + AO356_30365 0.31 +1.1 7,087,488 + AO356_30365 0.31 -0.1 7,087,488 + AO356_30365 0.31 -0.2 7,087,684 + AO356_30365 0.49 -0.1 7,087,752 - AO356_30365 0.56 -0.0 7,087,777 + AO356_30365 0.58 +0.6 7,087,777 + AO356_30365 0.58 +0.6 7,087,778 - AO356_30365 0.58 +0.6 7,087,778 - AO356_30365 0.58 +0.6 7,087,889 + AO356_30365 0.69 -0.1 7,087,890 - AO356_30365 0.69 +0.7 7,088,121 + +0.9 7,088,122 - -0.4 7,088,122 - +0.2 7,088,122 - -0.4 7,088,240 + +0.2 7,088,240 + +0.9 7,088,240 + +0.7 7,088,328 - -0.9 7,088,328 - -1.5 7,088,360 - -1.3 7,088,550 + AO356_30370 0.13 -0.0 7,088,550 + AO356_30370 0.13 +1.0 7,088,570 + AO356_30370 0.14 -0.3 7,088,571 - AO356_30370 0.14 -0.5 7,088,760 - AO356_30370 0.23 -0.1 7,088,805 - AO356_30370 0.25 -0.1 7,088,952 - AO356_30370 0.32 -0.7
Or see this region's nucleotide sequence