Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_21000

Experiment: Cholin acetate 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_20985 and AO356_20990 are separated by 72 nucleotidesAO356_20990 and AO356_20995 are separated by 30 nucleotidesAO356_20995 and AO356_21000 are separated by 238 nucleotidesAO356_21000 and AO356_21005 are separated by 153 nucleotidesAO356_21005 and AO356_21010 are separated by 118 nucleotidesAO356_21010 and AO356_21015 are separated by 154 nucleotides AO356_20985: AO356_20985 - 16S ribosomal RNA, at 4,670,688 to 4,672,233 _20985 AO356_20990: AO356_20990 - tRNA-Ile, at 4,672,306 to 4,672,382 _20990 AO356_20995: AO356_20995 - tRNA-Ala, at 4,672,413 to 4,672,488 _20995 AO356_21000: AO356_21000 - 23S ribosomal RNA, at 4,672,727 to 4,675,642 _21000 AO356_21005: AO356_21005 - 5S ribosomal RNA, at 4,675,796 to 4,675,911 _21005 AO356_21010: AO356_21010 - urease-associated protein, at 4,676,030 to 4,676,431 _21010 AO356_21015: AO356_21015 - vanillate O-demethylase oxidoreductase VanB, at 4,676,586 to 4,677,044 _21015 Position (kb) 4672 4673 4674 4675 4676Strain fitness (log2 ratio) -3 -2 -1 0 1at 4675.870 kb on + strand, within AO356_21005at 4675.968 kb on + strandat 4676.079 kb on + strand, within AO356_21010at 4676.079 kb on + strand, within AO356_21010at 4676.079 kb on + strand, within AO356_21010at 4676.080 kb on - strand, within AO356_21010at 4676.080 kb on - strand, within AO356_21010at 4676.080 kb on - strand, within AO356_21010at 4676.080 kb on - strand, within AO356_21010at 4676.080 kb on - strand, within AO356_21010at 4676.080 kb on - strand, within AO356_21010at 4676.133 kb on + strand, within AO356_21010at 4676.133 kb on + strand, within AO356_21010at 4676.133 kb on + strand, within AO356_21010at 4676.134 kb on - strand, within AO356_21010at 4676.146 kb on - strand, within AO356_21010at 4676.146 kb on - strand, within AO356_21010at 4676.280 kb on + strand, within AO356_21010at 4676.280 kb on + strand, within AO356_21010at 4676.280 kb on + strand, within AO356_21010at 4676.280 kb on + strand, within AO356_21010at 4676.281 kb on - strand, within AO356_21010at 4676.281 kb on - strand, within AO356_21010at 4676.350 kb on + strand, within AO356_21010at 4676.350 kb on + strand, within AO356_21010at 4676.350 kb on + strand, within AO356_21010at 4676.350 kb on + strand, within AO356_21010at 4676.350 kb on + strand, within AO356_21010at 4676.351 kb on - strand, within AO356_21010at 4676.351 kb on - strand, within AO356_21010at 4676.424 kb on - strandat 4676.473 kb on + strandat 4676.473 kb on + strandat 4676.473 kb on + strandat 4676.474 kb on - strandat 4676.474 kb on - strandat 4676.524 kb on + strandat 4676.525 kb on - strandat 4676.525 kb on - strandat 4676.552 kb on + strandat 4676.552 kb on + strandat 4676.552 kb on + strandat 4676.553 kb on - strandat 4676.553 kb on - strandat 4676.553 kb on - strandat 4676.553 kb on - strandat 4676.560 kb on + strandat 4676.560 kb on + strandat 4676.560 kb on + strandat 4676.561 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 40 mM
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4,675,870 + AO356_21005 0.64 -2.2
4,675,968 + +0.6
4,676,079 + AO356_21010 0.12 -1.4
4,676,079 + AO356_21010 0.12 -1.3
4,676,079 + AO356_21010 0.12 -0.8
4,676,080 - AO356_21010 0.12 -0.4
4,676,080 - AO356_21010 0.12 +1.5
4,676,080 - AO356_21010 0.12 -0.1
4,676,080 - AO356_21010 0.12 +0.5
4,676,080 - AO356_21010 0.12 +0.2
4,676,080 - AO356_21010 0.12 -0.4
4,676,133 + AO356_21010 0.26 -0.7
4,676,133 + AO356_21010 0.26 +0.9
4,676,133 + AO356_21010 0.26 +0.1
4,676,134 - AO356_21010 0.26 -0.5
4,676,146 - AO356_21010 0.29 +0.4
4,676,146 - AO356_21010 0.29 -0.7
4,676,280 + AO356_21010 0.62 +0.0
4,676,280 + AO356_21010 0.62 -0.8
4,676,280 + AO356_21010 0.62 -1.2
4,676,280 + AO356_21010 0.62 -0.6
4,676,281 - AO356_21010 0.62 +0.3
4,676,281 - AO356_21010 0.62 +0.3
4,676,350 + AO356_21010 0.80 +0.2
4,676,350 + AO356_21010 0.80 +0.7
4,676,350 + AO356_21010 0.80 +1.1
4,676,350 + AO356_21010 0.80 -0.8
4,676,350 + AO356_21010 0.80 -0.4
4,676,351 - AO356_21010 0.80 +0.2
4,676,351 - AO356_21010 0.80 +0.2
4,676,424 - +1.1
4,676,473 + +0.1
4,676,473 + +0.4
4,676,473 + -0.7
4,676,474 - +0.9
4,676,474 - -0.0
4,676,524 + +0.3
4,676,525 - -0.4
4,676,525 - -1.4
4,676,552 + -0.6
4,676,552 + +0.0
4,676,552 + +0.0
4,676,553 - -2.8
4,676,553 - +0.7
4,676,553 - -1.2
4,676,553 - -1.1
4,676,560 + +0.6
4,676,560 + +0.2
4,676,560 + -0.1
4,676,561 - +0.5

Or see this region's nucleotide sequence