Experiment: Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_13225 and AO356_13230 overlap by 8 nucleotides AO356_13230 and AO356_13235 are separated by 86 nucleotides AO356_13235 and AO356_13240 are separated by 116 nucleotides
AO356_13225: AO356_13225 - GTPase SAR1, at 3,006,365 to 3,007,738
_13225
AO356_13230: AO356_13230 - hypothetical protein, at 3,007,731 to 3,009,098
_13230
AO356_13235: AO356_13235 - diacylglycerol kinase, at 3,009,185 to 3,009,697
_13235
AO356_13240: AO356_13240 - L-cystine transporter tcyP, at 3,009,814 to 3,011,205
_13240
Position (kb)
3007
3008
3009
3010 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3006.903 kb on + strand, within AO356_13225 at 3006.904 kb on - strand, within AO356_13225 at 3007.152 kb on + strand, within AO356_13225 at 3007.152 kb on + strand, within AO356_13225 at 3007.153 kb on - strand, within AO356_13225 at 3007.153 kb on - strand, within AO356_13225 at 3007.153 kb on - strand, within AO356_13225 at 3007.153 kb on - strand, within AO356_13225 at 3007.332 kb on + strand, within AO356_13225 at 3007.332 kb on + strand, within AO356_13225 at 3007.333 kb on - strand, within AO356_13225 at 3007.333 kb on - strand, within AO356_13225 at 3007.333 kb on - strand, within AO356_13225 at 3007.368 kb on + strand, within AO356_13225 at 3007.369 kb on - strand, within AO356_13225 at 3007.369 kb on - strand, within AO356_13225 at 3007.369 kb on - strand, within AO356_13225 at 3007.369 kb on - strand, within AO356_13225 at 3007.369 kb on - strand, within AO356_13225 at 3007.369 kb on - strand, within AO356_13225 at 3007.369 kb on - strand, within AO356_13225 at 3007.369 kb on - strand, within AO356_13225 at 3007.512 kb on + strand, within AO356_13225 at 3007.512 kb on + strand, within AO356_13225 at 3007.512 kb on + strand, within AO356_13225 at 3007.554 kb on + strand, within AO356_13225 at 3007.554 kb on + strand, within AO356_13225 at 3007.554 kb on + strand, within AO356_13225 at 3007.555 kb on - strand, within AO356_13225 at 3007.555 kb on - strand, within AO356_13225 at 3007.555 kb on - strand, within AO356_13225 at 3007.555 kb on - strand, within AO356_13225 at 3007.667 kb on + strand at 3007.693 kb on + strand at 3007.694 kb on - strand at 3007.782 kb on + strand at 3007.783 kb on - strand at 3008.311 kb on + strand, within AO356_13230 at 3008.311 kb on + strand, within AO356_13230 at 3008.312 kb on - strand, within AO356_13230 at 3008.312 kb on - strand, within AO356_13230 at 3008.312 kb on - strand, within AO356_13230 at 3008.535 kb on + strand, within AO356_13230 at 3008.535 kb on + strand, within AO356_13230 at 3008.535 kb on + strand, within AO356_13230 at 3008.536 kb on - strand, within AO356_13230 at 3008.536 kb on - strand, within AO356_13230 at 3008.536 kb on - strand, within AO356_13230 at 3008.536 kb on - strand, within AO356_13230 at 3008.536 kb on - strand, within AO356_13230 at 3008.536 kb on - strand, within AO356_13230 at 3009.172 kb on + strand at 3009.695 kb on + strand at 3009.695 kb on + strand at 3009.696 kb on - strand at 3009.696 kb on - strand at 3009.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 40 mM remove 3,006,903 + AO356_13225 0.39 +0.1 3,006,904 - AO356_13225 0.39 -1.1 3,007,152 + AO356_13225 0.57 -0.4 3,007,152 + AO356_13225 0.57 -0.5 3,007,153 - AO356_13225 0.57 -0.0 3,007,153 - AO356_13225 0.57 +0.1 3,007,153 - AO356_13225 0.57 +0.3 3,007,153 - AO356_13225 0.57 +0.9 3,007,332 + AO356_13225 0.70 +0.6 3,007,332 + AO356_13225 0.70 +0.4 3,007,333 - AO356_13225 0.70 +0.5 3,007,333 - AO356_13225 0.70 -1.1 3,007,333 - AO356_13225 0.70 +0.7 3,007,368 + AO356_13225 0.73 -2.1 3,007,369 - AO356_13225 0.73 +0.7 3,007,369 - AO356_13225 0.73 -0.4 3,007,369 - AO356_13225 0.73 -0.7 3,007,369 - AO356_13225 0.73 -0.0 3,007,369 - AO356_13225 0.73 -0.7 3,007,369 - AO356_13225 0.73 +0.7 3,007,369 - AO356_13225 0.73 +1.3 3,007,369 - AO356_13225 0.73 -0.5 3,007,512 + AO356_13225 0.83 +0.7 3,007,512 + AO356_13225 0.83 +0.9 3,007,512 + AO356_13225 0.83 -0.0 3,007,554 + AO356_13225 0.87 +0.2 3,007,554 + AO356_13225 0.87 -2.1 3,007,554 + AO356_13225 0.87 +0.4 3,007,555 - AO356_13225 0.87 -1.7 3,007,555 - AO356_13225 0.87 -0.6 3,007,555 - AO356_13225 0.87 -1.2 3,007,555 - AO356_13225 0.87 -1.6 3,007,667 + +0.1 3,007,693 + +1.8 3,007,694 - -0.8 3,007,782 + +0.3 3,007,783 - +1.2 3,008,311 + AO356_13230 0.42 -0.5 3,008,311 + AO356_13230 0.42 -2.7 3,008,312 - AO356_13230 0.42 -0.9 3,008,312 - AO356_13230 0.42 +0.1 3,008,312 - AO356_13230 0.42 +0.6 3,008,535 + AO356_13230 0.59 -0.8 3,008,535 + AO356_13230 0.59 +0.3 3,008,535 + AO356_13230 0.59 -0.1 3,008,536 - AO356_13230 0.59 +0.0 3,008,536 - AO356_13230 0.59 +1.9 3,008,536 - AO356_13230 0.59 +0.3 3,008,536 - AO356_13230 0.59 +0.3 3,008,536 - AO356_13230 0.59 +0.1 3,008,536 - AO356_13230 0.59 +0.9 3,009,172 + +0.1 3,009,695 + +0.4 3,009,695 + -0.0 3,009,696 - +0.8 3,009,696 - +0.7 3,009,696 - +0.3
Or see this region's nucleotide sequence