Experiment: Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_05965 and AO356_05970 are separated by 201 nucleotides AO356_05970 and AO356_05975 overlap by 4 nucleotides AO356_05975 and AO356_05980 are separated by 2 nucleotides AO356_05980 and AO356_05985 overlap by 4 nucleotides
AO356_05965: AO356_05965 - DNA polymerase III subunit alpha, at 1,320,798 to 1,324,319
_05965
AO356_05970: AO356_05970 - ribonuclease HII, at 1,324,521 to 1,325,174
_05970
AO356_05975: AO356_05975 - lipid-A-disaccharide synthase, at 1,325,171 to 1,326,301
_05975
AO356_05980: AO356_05980 - UDP-N-acetylglucosamine acyltransferase, at 1,326,304 to 1,327,080
_05980
AO356_05985: AO356_05985 - 3-hydroxyacyl-ACP dehydratase, at 1,327,077 to 1,327,517
_05985
Position (kb)
1325
1326
1327 Strain fitness (log2 ratio)
-2
-1
0
1 at 1324.334 kb on - strand at 1324.363 kb on + strand at 1324.473 kb on - strand at 1324.564 kb on + strand at 1324.564 kb on + strand at 1324.565 kb on - strand at 1324.565 kb on - strand at 1324.565 kb on - strand at 1324.565 kb on - strand at 1324.565 kb on - strand at 1324.676 kb on - strand, within AO356_05970 at 1324.684 kb on + strand, within AO356_05970 at 1324.684 kb on + strand, within AO356_05970 at 1324.685 kb on - strand, within AO356_05970 at 1324.685 kb on - strand, within AO356_05970 at 1324.899 kb on - strand, within AO356_05970 at 1325.157 kb on + strand at 1325.157 kb on + strand at 1325.157 kb on + strand at 1325.157 kb on + strand at 1325.157 kb on + strand at 1325.157 kb on + strand at 1325.158 kb on - strand at 1325.158 kb on - strand at 1325.158 kb on - strand at 1325.163 kb on + strand at 1325.163 kb on + strand at 1325.163 kb on + strand at 1325.163 kb on + strand at 1325.163 kb on + strand at 1325.163 kb on + strand at 1325.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 40 mM remove 1,324,334 - +0.4 1,324,363 + -0.5 1,324,473 - -1.2 1,324,564 + -0.0 1,324,564 + +0.5 1,324,565 - +0.9 1,324,565 - -0.3 1,324,565 - -0.8 1,324,565 - +1.1 1,324,565 - +1.5 1,324,676 - AO356_05970 0.24 +0.9 1,324,684 + AO356_05970 0.25 +0.6 1,324,684 + AO356_05970 0.25 -0.4 1,324,685 - AO356_05970 0.25 +0.6 1,324,685 - AO356_05970 0.25 -0.7 1,324,899 - AO356_05970 0.58 -0.1 1,325,157 + +1.0 1,325,157 + -0.2 1,325,157 + -1.9 1,325,157 + +0.3 1,325,157 + -1.1 1,325,157 + -0.9 1,325,158 - +0.1 1,325,158 - -0.1 1,325,158 - +0.7 1,325,163 + -0.0 1,325,163 + -0.4 1,325,163 + +0.5 1,325,163 + -1.1 1,325,163 + +1.4 1,325,163 + -0.4 1,325,164 - +0.3
Or see this region's nucleotide sequence