Experiment: Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02225 and AO356_02230 are separated by 63 nucleotides AO356_02230 and AO356_02235 overlap by 8 nucleotides AO356_02235 and AO356_02240 are separated by 94 nucleotides AO356_02240 and AO356_02245 overlap by 4 nucleotides
AO356_02225: AO356_02225 - multidrug ABC transporter substrate-binding protein, at 477,187 to 478,431
_02225
AO356_02230: AO356_02230 - ABC transporter, at 478,495 to 479,193
_02230
AO356_02235: AO356_02235 - cell division protein FtsX, at 479,186 to 480,436
_02235
AO356_02240: AO356_02240 - pilus assembly protein PilZ, at 480,531 to 481,127
_02240
AO356_02245: AO356_02245 - glycerophosphodiester phosphodiesterase, at 481,124 to 481,846
_02245
Position (kb) 479
480
481 Strain fitness (log2 ratio) -1
0
1 at 480.497 kb on - strand at 480.497 kb on - strand at 480.497 kb on - strand at 480.497 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.565 kb on - strand at 480.750 kb on + strand, within AO356_02240 at 480.917 kb on - strand, within AO356_02240 at 481.047 kb on + strand, within AO356_02240 at 481.048 kb on - strand, within AO356_02240 at 481.106 kb on + strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.112 kb on + strand at 481.136 kb on + strand at 481.136 kb on + strand at 481.136 kb on + strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.212 kb on + strand, within AO356_02245 at 481.213 kb on - strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.344 kb on - strand, within AO356_02245 at 481.344 kb on - strand, within AO356_02245
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 40 mM remove 480,497 - -0.8 480,497 - +0.7 480,497 - +0.4 480,497 - -0.4 480,542 - -0.7 480,542 - -0.1 480,542 - +1.5 480,542 - +1.0 480,565 - +0.1 480,750 + AO356_02240 0.37 -1.0 480,917 - AO356_02240 0.65 +0.8 481,047 + AO356_02240 0.86 +0.3 481,048 - AO356_02240 0.87 +0.0 481,106 + -0.1 481,107 - -1.6 481,107 - +0.3 481,107 - +0.3 481,107 - -0.6 481,112 + -0.1 481,136 + -0.2 481,136 + +0.5 481,136 + -0.3 481,137 - -0.3 481,137 - -0.2 481,137 - -1.2 481,137 - -1.3 481,137 - -0.8 481,212 + AO356_02245 0.12 -0.6 481,213 - AO356_02245 0.12 +0.1 481,343 + AO356_02245 0.30 +0.6 481,343 + AO356_02245 0.30 +1.0 481,343 + AO356_02245 0.30 +1.5 481,343 + AO356_02245 0.30 +0.0 481,344 - AO356_02245 0.30 -0.2 481,344 - AO356_02245 0.30 +1.1
Or see this region's nucleotide sequence