Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2964 and Dshi_2965 are separated by 84 nucleotides Dshi_2965 and Dshi_2966 are separated by 168 nucleotides Dshi_2966 and Dshi_2967 are separated by 236 nucleotides
Dshi_2964: Dshi_2964 - basic membrane lipoprotein (RefSeq), at 3,130,346 to 3,131,509
_2964
Dshi_2965: Dshi_2965 - hypothetical protein (RefSeq), at 3,131,594 to 3,131,788
_2965
Dshi_2966: Dshi_2966 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (RefSeq), at 3,131,957 to 3,132,778
_2966
Dshi_2967: Dshi_2967 - FAD dependent oxidoreductase (RefSeq), at 3,133,015 to 3,135,510
_2967
Position (kb)
3131
3132
3133 Strain fitness (log2 ratio)
-2
-1
0
1 at 3130.969 kb on - strand, within Dshi_2964 at 3131.369 kb on + strand, within Dshi_2964 at 3131.380 kb on + strand, within Dshi_2964 at 3131.381 kb on + strand, within Dshi_2964 at 3131.470 kb on + strand at 3131.570 kb on + strand at 3131.616 kb on + strand, within Dshi_2965 at 3131.634 kb on + strand, within Dshi_2965 at 3131.651 kb on - strand, within Dshi_2965 at 3131.747 kb on + strand, within Dshi_2965 at 3131.798 kb on - strand at 3131.846 kb on - strand at 3131.846 kb on - strand at 3131.915 kb on + strand at 3132.872 kb on + strand at 3132.954 kb on - strand at 3132.975 kb on + strand at 3132.998 kb on - strand at 3133.008 kb on - strand at 3133.073 kb on + strand at 3133.178 kb on - strand at 3133.228 kb on + strand at 3133.229 kb on - strand at 3133.236 kb on - strand at 3133.267 kb on + strand, within Dshi_2967 at 3133.288 kb on + strand, within Dshi_2967 at 3133.288 kb on + strand, within Dshi_2967 at 3133.296 kb on - strand, within Dshi_2967 at 3133.476 kb on + strand, within Dshi_2967 at 3133.482 kb on + strand, within Dshi_2967 at 3133.634 kb on - strand, within Dshi_2967
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 3,130,969 - Dshi_2964 0.54 -0.1 3,131,369 + Dshi_2964 0.88 -1.4 3,131,380 + Dshi_2964 0.89 -0.2 3,131,381 + Dshi_2964 0.89 +0.0 3,131,470 + +0.1 3,131,570 + -1.2 3,131,616 + Dshi_2965 0.11 -0.5 3,131,634 + Dshi_2965 0.21 -1.1 3,131,651 - Dshi_2965 0.29 -0.1 3,131,747 + Dshi_2965 0.78 -0.1 3,131,798 - -0.8 3,131,846 - -1.8 3,131,846 - -0.1 3,131,915 + -0.5 3,132,872 + -0.9 3,132,954 - +0.1 3,132,975 + -0.4 3,132,998 - +1.0 3,133,008 - +0.0 3,133,073 + -0.4 3,133,178 - -0.3 3,133,228 + -0.2 3,133,229 - +0.7 3,133,236 - -0.4 3,133,267 + Dshi_2967 0.10 -0.8 3,133,288 + Dshi_2967 0.11 +0.3 3,133,288 + Dshi_2967 0.11 -0.7 3,133,296 - Dshi_2967 0.11 +0.1 3,133,476 + Dshi_2967 0.18 -0.4 3,133,482 + Dshi_2967 0.19 -1.6 3,133,634 - Dshi_2967 0.25 -0.9
Or see this region's nucleotide sequence