Experiment: Bile salts 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3035 and BT3036 are separated by 80 nucleotides BT3036 and BT3037 are separated by 207 nucleotides BT3037 and BT3038 are separated by 16 nucleotides
BT3035: BT3035 - carboxy-terminal processing protease precursor (NCBI ptt file), at 3,842,449 to 3,844,038
BT3035
BT3036: BT3036 - conserved hypothetical protein (NCBI ptt file), at 3,844,119 to 3,846,701
BT3036
BT3037: BT3037 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 3,846,909 to 3,847,418
BT3037
BT3038: BT3038 - putative anti-sigma factor (NCBI ptt file), at 3,847,435 to 3,848,475
BT3038
Position (kb)
3844
3845
3846
3847 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3843.148 kb on + strand, within BT3035 at 3843.302 kb on - strand, within BT3035 at 3843.357 kb on + strand, within BT3035 at 3843.365 kb on + strand, within BT3035 at 3843.451 kb on + strand, within BT3035 at 3843.452 kb on - strand, within BT3035 at 3843.455 kb on - strand, within BT3035 at 3843.506 kb on - strand, within BT3035 at 3843.510 kb on + strand, within BT3035 at 3843.521 kb on + strand, within BT3035 at 3843.522 kb on - strand, within BT3035 at 3843.522 kb on - strand, within BT3035 at 3843.660 kb on + strand, within BT3035 at 3843.670 kb on + strand, within BT3035 at 3843.762 kb on + strand, within BT3035 at 3843.766 kb on + strand, within BT3035 at 3843.766 kb on + strand, within BT3035 at 3843.967 kb on - strand at 3843.967 kb on - strand at 3844.089 kb on + strand at 3844.090 kb on - strand at 3844.090 kb on - strand at 3844.093 kb on - strand at 3844.099 kb on - strand at 3844.118 kb on + strand at 3844.118 kb on + strand at 3844.124 kb on - strand at 3844.124 kb on - strand at 3844.425 kb on + strand, within BT3036 at 3844.480 kb on + strand, within BT3036 at 3844.481 kb on - strand, within BT3036 at 3844.482 kb on + strand, within BT3036 at 3844.629 kb on + strand, within BT3036 at 3844.630 kb on - strand, within BT3036 at 3844.633 kb on + strand, within BT3036 at 3844.633 kb on + strand, within BT3036 at 3844.634 kb on - strand, within BT3036 at 3844.714 kb on + strand, within BT3036 at 3844.714 kb on + strand, within BT3036 at 3844.715 kb on - strand, within BT3036 at 3844.742 kb on - strand, within BT3036 at 3844.775 kb on - strand, within BT3036 at 3844.892 kb on - strand, within BT3036 at 3844.952 kb on - strand, within BT3036 at 3844.952 kb on - strand, within BT3036 at 3845.110 kb on + strand at 3845.121 kb on + strand, within BT3036 at 3845.121 kb on + strand, within BT3036 at 3845.121 kb on + strand, within BT3036 at 3845.121 kb on + strand, within BT3036 at 3845.208 kb on + strand, within BT3036 at 3845.208 kb on + strand, within BT3036 at 3845.322 kb on - strand, within BT3036 at 3845.328 kb on + strand, within BT3036 at 3845.328 kb on + strand, within BT3036 at 3845.328 kb on + strand, within BT3036 at 3845.329 kb on - strand, within BT3036 at 3845.329 kb on - strand, within BT3036 at 3845.330 kb on + strand, within BT3036 at 3845.331 kb on - strand, within BT3036 at 3845.360 kb on + strand, within BT3036 at 3845.360 kb on + strand, within BT3036 at 3845.361 kb on - strand, within BT3036 at 3845.361 kb on - strand, within BT3036 at 3845.361 kb on - strand, within BT3036 at 3845.363 kb on + strand, within BT3036 at 3845.364 kb on - strand, within BT3036 at 3845.476 kb on - strand, within BT3036 at 3845.520 kb on + strand, within BT3036 at 3845.527 kb on - strand, within BT3036 at 3845.611 kb on - strand, within BT3036 at 3845.611 kb on - strand, within BT3036 at 3845.698 kb on + strand, within BT3036 at 3845.699 kb on - strand, within BT3036 at 3845.914 kb on - strand, within BT3036 at 3845.962 kb on - strand, within BT3036 at 3845.974 kb on - strand, within BT3036 at 3846.000 kb on + strand, within BT3036 at 3846.001 kb on - strand, within BT3036 at 3846.013 kb on - strand, within BT3036 at 3846.052 kb on + strand, within BT3036 at 3846.109 kb on - strand, within BT3036 at 3846.109 kb on - strand, within BT3036 at 3846.125 kb on + strand, within BT3036 at 3846.129 kb on + strand, within BT3036 at 3846.129 kb on + strand, within BT3036 at 3846.129 kb on + strand, within BT3036 at 3846.130 kb on - strand, within BT3036 at 3846.130 kb on - strand, within BT3036 at 3846.175 kb on + strand, within BT3036 at 3846.175 kb on + strand, within BT3036 at 3846.253 kb on + strand, within BT3036 at 3846.254 kb on - strand, within BT3036 at 3846.311 kb on - strand, within BT3036 at 3846.311 kb on - strand, within BT3036 at 3846.311 kb on - strand, within BT3036 at 3846.433 kb on - strand, within BT3036 at 3846.549 kb on + strand at 3846.561 kb on + strand at 3846.657 kb on + strand at 3846.658 kb on - strand at 3846.659 kb on + strand at 3846.758 kb on - strand at 3846.768 kb on + strand at 3846.768 kb on + strand at 3846.769 kb on - strand at 3846.769 kb on - strand at 3846.771 kb on + strand at 3846.772 kb on - strand at 3846.823 kb on - strand at 3846.878 kb on + strand at 3846.904 kb on - strand at 3846.991 kb on - strand, within BT3037 at 3847.107 kb on + strand, within BT3037 at 3847.110 kb on - strand, within BT3037 at 3847.182 kb on + strand, within BT3037 at 3847.183 kb on - strand, within BT3037 at 3847.211 kb on + strand, within BT3037 at 3847.298 kb on - strand, within BT3037 at 3847.302 kb on + strand, within BT3037 at 3847.302 kb on + strand, within BT3037 at 3847.376 kb on - strand at 3847.391 kb on + strand at 3847.392 kb on - strand at 3847.430 kb on - strand at 3847.534 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.25 mg/ml remove 3,843,148 + BT3035 0.44 -2.9 3,843,302 - BT3035 0.54 -0.3 3,843,357 + BT3035 0.57 -4.3 3,843,365 + BT3035 0.58 -1.7 3,843,451 + BT3035 0.63 -2.1 3,843,452 - BT3035 0.63 -2.6 3,843,455 - BT3035 0.63 -2.9 3,843,506 - BT3035 0.66 -0.7 3,843,510 + BT3035 0.67 -5.5 3,843,521 + BT3035 0.67 -1.9 3,843,522 - BT3035 0.67 -3.5 3,843,522 - BT3035 0.67 -2.4 3,843,660 + BT3035 0.76 -1.0 3,843,670 + BT3035 0.77 -1.7 3,843,762 + BT3035 0.83 -3.2 3,843,766 + BT3035 0.83 -1.9 3,843,766 + BT3035 0.83 -2.6 3,843,967 - -2.5 3,843,967 - -2.1 3,844,089 + -1.4 3,844,090 - +0.6 3,844,090 - +0.7 3,844,093 - -0.3 3,844,099 - +0.6 3,844,118 + -0.3 3,844,118 + -0.8 3,844,124 - -3.0 3,844,124 - -1.9 3,844,425 + BT3036 0.12 -1.1 3,844,480 + BT3036 0.14 -0.3 3,844,481 - BT3036 0.14 -0.1 3,844,482 + BT3036 0.14 -0.2 3,844,629 + BT3036 0.20 -2.1 3,844,630 - BT3036 0.20 +0.3 3,844,633 + BT3036 0.20 +0.1 3,844,633 + BT3036 0.20 -0.6 3,844,634 - BT3036 0.20 -0.3 3,844,714 + BT3036 0.23 -1.7 3,844,714 + BT3036 0.23 -1.0 3,844,715 - BT3036 0.23 +0.3 3,844,742 - BT3036 0.24 -2.8 3,844,775 - BT3036 0.25 -1.2 3,844,892 - BT3036 0.30 +2.3 3,844,952 - BT3036 0.32 +0.3 3,844,952 - BT3036 0.32 -1.3 3,845,110 + +1.3 3,845,121 + BT3036 0.39 -0.0 3,845,121 + BT3036 0.39 +0.8 3,845,121 + BT3036 0.39 -0.8 3,845,121 + BT3036 0.39 -0.7 3,845,208 + BT3036 0.42 +0.7 3,845,208 + BT3036 0.42 -0.1 3,845,322 - BT3036 0.47 -0.3 3,845,328 + BT3036 0.47 -1.0 3,845,328 + BT3036 0.47 +0.1 3,845,328 + BT3036 0.47 -0.3 3,845,329 - BT3036 0.47 -1.1 3,845,329 - BT3036 0.47 -1.7 3,845,330 + BT3036 0.47 -0.7 3,845,331 - BT3036 0.47 +1.3 3,845,360 + BT3036 0.48 -0.8 3,845,360 + BT3036 0.48 -0.6 3,845,361 - BT3036 0.48 -0.5 3,845,361 - BT3036 0.48 -0.0 3,845,361 - BT3036 0.48 -0.7 3,845,363 + BT3036 0.48 -0.5 3,845,364 - BT3036 0.48 -2.8 3,845,476 - BT3036 0.53 -2.0 3,845,520 + BT3036 0.54 -0.0 3,845,527 - BT3036 0.55 +0.9 3,845,611 - BT3036 0.58 -1.0 3,845,611 - BT3036 0.58 -0.4 3,845,698 + BT3036 0.61 -1.3 3,845,699 - BT3036 0.61 -1.5 3,845,914 - BT3036 0.69 -0.2 3,845,962 - BT3036 0.71 -2.3 3,845,974 - BT3036 0.72 +0.3 3,846,000 + BT3036 0.73 -0.1 3,846,001 - BT3036 0.73 -1.2 3,846,013 - BT3036 0.73 +0.6 3,846,052 + BT3036 0.75 -1.2 3,846,109 - BT3036 0.77 -1.1 3,846,109 - BT3036 0.77 -0.7 3,846,125 + BT3036 0.78 -0.9 3,846,129 + BT3036 0.78 +0.9 3,846,129 + BT3036 0.78 -1.6 3,846,129 + BT3036 0.78 -0.4 3,846,130 - BT3036 0.78 -1.4 3,846,130 - BT3036 0.78 +0.3 3,846,175 + BT3036 0.80 -1.7 3,846,175 + BT3036 0.80 -1.9 3,846,253 + BT3036 0.83 -0.3 3,846,254 - BT3036 0.83 -1.3 3,846,311 - BT3036 0.85 -0.3 3,846,311 - BT3036 0.85 -0.5 3,846,311 - BT3036 0.85 -1.3 3,846,433 - BT3036 0.90 -1.5 3,846,549 + -0.7 3,846,561 + -0.2 3,846,657 + -1.9 3,846,658 - -0.6 3,846,659 + +0.7 3,846,758 - -0.7 3,846,768 + -2.8 3,846,768 + -1.3 3,846,769 - -1.7 3,846,769 - -2.0 3,846,771 + -1.6 3,846,772 - -0.9 3,846,823 - +0.5 3,846,878 + -3.3 3,846,904 - -1.7 3,846,991 - BT3037 0.16 -0.7 3,847,107 + BT3037 0.39 +0.5 3,847,110 - BT3037 0.39 +0.1 3,847,182 + BT3037 0.54 -0.3 3,847,183 - BT3037 0.54 +0.7 3,847,211 + BT3037 0.59 -1.9 3,847,298 - BT3037 0.76 -0.5 3,847,302 + BT3037 0.77 -1.1 3,847,302 + BT3037 0.77 -0.9 3,847,376 - -1.1 3,847,391 + -1.8 3,847,392 - -0.8 3,847,430 - -1.8 3,847,534 - +1.3
Or see this region's nucleotide sequence