Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27860

Experiment: D,L-Malic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27855 and BWI76_RS27860 are separated by 17 nucleotidesBWI76_RS27860 and BWI76_RS27865 are separated by 53 nucleotidesBWI76_RS27865 and BWI76_RS27870 overlap by 4 nucleotidesBWI76_RS27870 and BWI76_RS27875 overlap by 11 nucleotides BWI76_RS27855: BWI76_RS27855 - D-serine transporter DsdX, at 5,777,380 to 5,778,717 _RS27855 BWI76_RS27860: BWI76_RS27860 - D-serine dehydratase, at 5,778,735 to 5,780,063 _RS27860 BWI76_RS27865: BWI76_RS27865 - hypothetical protein, at 5,780,117 to 5,780,647 _RS27865 BWI76_RS27870: BWI76_RS27870 - membrane protein, at 5,780,644 to 5,781,006 _RS27870 BWI76_RS27875: BWI76_RS27875 - membrane protein, at 5,780,996 to 5,781,343 _RS27875 Position (kb) 5778 5779 5780 5781Strain fitness (log2 ratio) -3 -2 -1 0 1at 5777.828 kb on - strand, within BWI76_RS27855at 5777.997 kb on + strand, within BWI76_RS27855at 5778.075 kb on + strandat 5778.075 kb on + strand, within BWI76_RS27855at 5778.075 kb on + strand, within BWI76_RS27855at 5778.075 kb on + strand, within BWI76_RS27855at 5778.098 kb on + strand, within BWI76_RS27855at 5778.574 kb on + strand, within BWI76_RS27855at 5778.854 kb on - strandat 5778.854 kb on - strandat 5779.033 kb on + strand, within BWI76_RS27860at 5779.167 kb on - strand, within BWI76_RS27860at 5779.214 kb on + strand, within BWI76_RS27860at 5779.222 kb on - strand, within BWI76_RS27860at 5779.290 kb on - strand, within BWI76_RS27860at 5779.296 kb on + strand, within BWI76_RS27860at 5779.296 kb on + strand, within BWI76_RS27860at 5779.304 kb on - strand, within BWI76_RS27860at 5779.384 kb on - strand, within BWI76_RS27860at 5779.417 kb on + strand, within BWI76_RS27860at 5779.503 kb on - strand, within BWI76_RS27860at 5779.692 kb on - strand, within BWI76_RS27860at 5779.811 kb on + strand, within BWI76_RS27860at 5779.841 kb on - strand, within BWI76_RS27860at 5779.912 kb on + strand, within BWI76_RS27860at 5779.957 kb on + strandat 5779.976 kb on - strandat 5780.042 kb on + strandat 5780.143 kb on - strandat 5780.233 kb on + strand, within BWI76_RS27865at 5780.289 kb on - strand, within BWI76_RS27865at 5780.375 kb on - strand, within BWI76_RS27865at 5780.375 kb on - strand, within BWI76_RS27865at 5780.417 kb on + strand, within BWI76_RS27865at 5780.600 kb on - strandat 5780.679 kb on + strandat 5780.693 kb on - strand, within BWI76_RS27870at 5780.775 kb on + strand, within BWI76_RS27870at 5780.917 kb on + strand, within BWI76_RS27870at 5780.917 kb on + strand, within BWI76_RS27870at 5780.925 kb on - strand, within BWI76_RS27870at 5781.046 kb on + strand, within BWI76_RS27875

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Malic Acid (C)
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5,777,828 - BWI76_RS27855 0.33 -0.5
5,777,997 + BWI76_RS27855 0.46 -0.3
5,778,075 + +1.5
5,778,075 + BWI76_RS27855 0.52 +0.3
5,778,075 + BWI76_RS27855 0.52 +1.2
5,778,075 + BWI76_RS27855 0.52 -0.3
5,778,098 + BWI76_RS27855 0.54 -0.3
5,778,574 + BWI76_RS27855 0.89 +0.5
5,778,854 - +0.3
5,778,854 - -1.8
5,779,033 + BWI76_RS27860 0.22 +0.2
5,779,167 - BWI76_RS27860 0.33 -0.8
5,779,214 + BWI76_RS27860 0.36 +0.2
5,779,222 - BWI76_RS27860 0.37 -0.9
5,779,290 - BWI76_RS27860 0.42 -0.8
5,779,296 + BWI76_RS27860 0.42 -2.1
5,779,296 + BWI76_RS27860 0.42 -2.7
5,779,304 - BWI76_RS27860 0.43 -2.3
5,779,384 - BWI76_RS27860 0.49 -2.7
5,779,417 + BWI76_RS27860 0.51 -1.5
5,779,503 - BWI76_RS27860 0.58 -1.4
5,779,692 - BWI76_RS27860 0.72 -3.1
5,779,811 + BWI76_RS27860 0.81 -2.2
5,779,841 - BWI76_RS27860 0.83 +0.5
5,779,912 + BWI76_RS27860 0.89 -3.7
5,779,957 + +0.3
5,779,976 - -1.6
5,780,042 + -0.5
5,780,143 - +0.1
5,780,233 + BWI76_RS27865 0.22 +0.5
5,780,289 - BWI76_RS27865 0.32 -0.1
5,780,375 - BWI76_RS27865 0.49 -2.3
5,780,375 - BWI76_RS27865 0.49 +0.3
5,780,417 + BWI76_RS27865 0.56 +1.5
5,780,600 - -0.4
5,780,679 + -0.9
5,780,693 - BWI76_RS27870 0.13 +0.1
5,780,775 + BWI76_RS27870 0.36 +0.0
5,780,917 + BWI76_RS27870 0.75 -0.5
5,780,917 + BWI76_RS27870 0.75 -0.1
5,780,925 - BWI76_RS27870 0.77 +0.2
5,781,046 + BWI76_RS27875 0.14 +0.3

Or see this region's nucleotide sequence