Experiment: D,L-Malic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27195 and BWI76_RS27200 are separated by 49 nucleotides BWI76_RS27200 and BWI76_RS27205 are separated by 105 nucleotides BWI76_RS27205 and BWI76_RS27210 are separated by 80 nucleotides
BWI76_RS27195: BWI76_RS27195 - alpha-hydroxy-acid oxidizing enzyme, at 5,645,418 to 5,646,648
_RS27195
BWI76_RS27200: BWI76_RS27200 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 5,646,698 to 5,647,171
_RS27200
BWI76_RS27205: BWI76_RS27205 - serine O-acetyltransferase, at 5,647,277 to 5,648,098
_RS27205
BWI76_RS27210: BWI76_RS27210 - glycerol-3-phosphate dehydrogenase, at 5,648,179 to 5,649,195
_RS27210
Position (kb) 5647
5648
5649 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5646.406 kb on + strand, within BWI76_RS27195 at 5646.651 kb on - strand at 5646.652 kb on - strand at 5646.866 kb on + strand, within BWI76_RS27200 at 5646.924 kb on - strand, within BWI76_RS27200 at 5646.966 kb on + strand, within BWI76_RS27200 at 5647.005 kb on + strand, within BWI76_RS27200 at 5647.322 kb on + strand at 5648.103 kb on - strand at 5648.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) remove 5,646,406 + BWI76_RS27195 0.80 +0.8 5,646,651 - -0.9 5,646,652 - -0.8 5,646,866 + BWI76_RS27200 0.35 -0.3 5,646,924 - BWI76_RS27200 0.48 -0.4 5,646,966 + BWI76_RS27200 0.57 +1.7 5,647,005 + BWI76_RS27200 0.65 -0.8 5,647,322 + -4.7 5,648,103 - -4.3 5,648,103 - -0.5
Or see this region's nucleotide sequence