Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25980

Experiment: D,L-Malic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25970 and BWI76_RS25975 are separated by 35 nucleotidesBWI76_RS25975 and BWI76_RS25980 are separated by 46 nucleotidesBWI76_RS25980 and BWI76_RS25985 overlap by 41 nucleotidesBWI76_RS25985 and BWI76_RS25990 are separated by 157 nucleotidesBWI76_RS25990 and BWI76_RS25995 are separated by 51 nucleotides BWI76_RS25970: BWI76_RS25970 - hypothetical protein, at 5,369,216 to 5,369,434 _RS25970 BWI76_RS25975: BWI76_RS25975 - phosphoribulokinase, at 5,369,470 to 5,370,339 _RS25975 BWI76_RS25980: BWI76_RS25980 - hypothetical protein, at 5,370,386 to 5,370,790 _RS25980 BWI76_RS25985: BWI76_RS25985 - hypothetical protein, at 5,370,750 to 5,370,937 _RS25985 BWI76_RS25990: BWI76_RS25990 - cAMP-activated global transcriptional regulator CRP, at 5,371,095 to 5,371,727 _RS25990 BWI76_RS25995: BWI76_RS25995 - membrane protein, at 5,371,779 to 5,373,857 _RS25995 Position (kb) 5370 5371Strain fitness (log2 ratio) -2 -1 0 1at 5369.392 kb on - strand, within BWI76_RS25970at 5369.392 kb on - strand, within BWI76_RS25970at 5369.503 kb on + strandat 5369.503 kb on + strandat 5369.711 kb on - strand, within BWI76_RS25975at 5369.746 kb on - strand, within BWI76_RS25975at 5369.868 kb on - strand, within BWI76_RS25975at 5370.107 kb on + strand, within BWI76_RS25975at 5370.115 kb on - strand, within BWI76_RS25975at 5370.493 kb on - strand, within BWI76_RS25980at 5370.493 kb on - strand, within BWI76_RS25980at 5370.569 kb on + strand, within BWI76_RS25980at 5370.968 kb on + strandat 5370.971 kb on - strandat 5370.992 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Malic Acid (C)
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5,369,392 - BWI76_RS25970 0.80 -0.4
5,369,392 - BWI76_RS25970 0.80 -0.7
5,369,503 + +1.5
5,369,503 + -1.3
5,369,711 - BWI76_RS25975 0.28 -1.2
5,369,746 - BWI76_RS25975 0.32 -1.8
5,369,868 - BWI76_RS25975 0.46 -1.4
5,370,107 + BWI76_RS25975 0.73 -1.5
5,370,115 - BWI76_RS25975 0.74 -2.2
5,370,493 - BWI76_RS25980 0.26 +0.5
5,370,493 - BWI76_RS25980 0.26 -0.0
5,370,569 + BWI76_RS25980 0.45 -0.2
5,370,968 + -1.0
5,370,971 - -2.7
5,370,992 + -0.9

Or see this region's nucleotide sequence