Experiment: D,L-Malic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23860 and BWI76_RS23865 are separated by 186 nucleotides BWI76_RS23865 and BWI76_RS23870 are separated by 60 nucleotides BWI76_RS23870 and BWI76_RS23875 are separated by 144 nucleotides BWI76_RS23875 and BWI76_RS23880 are separated by 22 nucleotides
BWI76_RS23860: BWI76_RS23860 - protein disulfide oxidoreductase, at 4,978,989 to 4,979,480
_RS23860
BWI76_RS23865: BWI76_RS23865 - NAD(P)-dependent oxidoreductase, at 4,979,667 to 4,980,410
_RS23865
BWI76_RS23870: BWI76_RS23870 - Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data) , at 4,980,471 to 4,983,344
_RS23870
BWI76_RS23875: BWI76_RS23875 - glycine cleavage system protein H, at 4,983,489 to 4,983,878
_RS23875
BWI76_RS23880: BWI76_RS23880 - Aminomethyltransferase (EC 2.1.2.10) (from data) , at 4,983,901 to 4,984,995
_RS23880
Position (kb)
4980
4981
4982
4983
4984 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4979.524 kb on + strand at 4979.556 kb on - strand at 4979.580 kb on + strand at 4979.590 kb on + strand at 4979.598 kb on - strand at 4979.606 kb on - strand at 4979.844 kb on + strand, within BWI76_RS23865 at 4980.004 kb on + strand, within BWI76_RS23865 at 4980.012 kb on - strand, within BWI76_RS23865 at 4980.038 kb on + strand, within BWI76_RS23865 at 4980.154 kb on + strand, within BWI76_RS23865 at 4980.162 kb on - strand, within BWI76_RS23865 at 4980.213 kb on + strand, within BWI76_RS23865 at 4980.309 kb on - strand, within BWI76_RS23865 at 4980.309 kb on - strand, within BWI76_RS23865 at 4980.339 kb on - strand at 4980.564 kb on + strand at 4980.778 kb on - strand, within BWI76_RS23870 at 4981.058 kb on + strand, within BWI76_RS23870 at 4981.219 kb on + strand, within BWI76_RS23870 at 4981.236 kb on - strand, within BWI76_RS23870 at 4981.273 kb on + strand, within BWI76_RS23870 at 4981.679 kb on + strand, within BWI76_RS23870 at 4981.905 kb on + strand, within BWI76_RS23870 at 4981.989 kb on - strand, within BWI76_RS23870 at 4982.075 kb on + strand, within BWI76_RS23870 at 4982.133 kb on - strand, within BWI76_RS23870 at 4982.162 kb on + strand, within BWI76_RS23870 at 4982.182 kb on - strand, within BWI76_RS23870 at 4982.185 kb on - strand, within BWI76_RS23870 at 4982.395 kb on + strand, within BWI76_RS23870 at 4982.405 kb on + strand, within BWI76_RS23870 at 4982.408 kb on - strand, within BWI76_RS23870 at 4982.410 kb on + strand, within BWI76_RS23870 at 4982.529 kb on + strand, within BWI76_RS23870 at 4982.685 kb on - strand, within BWI76_RS23870 at 4983.048 kb on - strand, within BWI76_RS23870 at 4983.084 kb on - strand at 4983.250 kb on + strand at 4983.269 kb on + strand at 4983.334 kb on - strand at 4983.461 kb on + strand at 4983.608 kb on + strand, within BWI76_RS23875 at 4983.608 kb on + strand, within BWI76_RS23875 at 4983.616 kb on - strand at 4983.616 kb on - strand, within BWI76_RS23875 at 4983.719 kb on + strand, within BWI76_RS23875 at 4983.737 kb on + strand, within BWI76_RS23875 at 4984.179 kb on + strand, within BWI76_RS23880 at 4984.294 kb on + strand, within BWI76_RS23880
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) remove 4,979,524 + -0.5 4,979,556 - +0.6 4,979,580 + +0.3 4,979,590 + +0.7 4,979,598 - +0.8 4,979,606 - +0.0 4,979,844 + BWI76_RS23865 0.24 +0.1 4,980,004 + BWI76_RS23865 0.45 +0.2 4,980,012 - BWI76_RS23865 0.46 +0.7 4,980,038 + BWI76_RS23865 0.50 -0.1 4,980,154 + BWI76_RS23865 0.65 -0.3 4,980,162 - BWI76_RS23865 0.67 +1.4 4,980,213 + BWI76_RS23865 0.73 -0.1 4,980,309 - BWI76_RS23865 0.86 -0.9 4,980,309 - BWI76_RS23865 0.86 -0.1 4,980,339 - +0.9 4,980,564 + -0.3 4,980,778 - BWI76_RS23870 0.11 -0.6 4,981,058 + BWI76_RS23870 0.20 -0.5 4,981,219 + BWI76_RS23870 0.26 +0.5 4,981,236 - BWI76_RS23870 0.27 -0.3 4,981,273 + BWI76_RS23870 0.28 -0.4 4,981,679 + BWI76_RS23870 0.42 -0.3 4,981,905 + BWI76_RS23870 0.50 -1.5 4,981,989 - BWI76_RS23870 0.53 -0.8 4,982,075 + BWI76_RS23870 0.56 -0.5 4,982,133 - BWI76_RS23870 0.58 -1.1 4,982,162 + BWI76_RS23870 0.59 -1.5 4,982,182 - BWI76_RS23870 0.60 +0.8 4,982,185 - BWI76_RS23870 0.60 +0.1 4,982,395 + BWI76_RS23870 0.67 -0.6 4,982,405 + BWI76_RS23870 0.67 -0.4 4,982,408 - BWI76_RS23870 0.67 -0.8 4,982,410 + BWI76_RS23870 0.67 -0.6 4,982,529 + BWI76_RS23870 0.72 -0.3 4,982,685 - BWI76_RS23870 0.77 -1.0 4,983,048 - BWI76_RS23870 0.90 +2.9 4,983,084 - -0.4 4,983,250 + -0.7 4,983,269 + -0.3 4,983,334 - -0.4 4,983,461 + -0.4 4,983,608 + BWI76_RS23875 0.31 -0.6 4,983,608 + BWI76_RS23875 0.31 -0.6 4,983,616 - +2.4 4,983,616 - BWI76_RS23875 0.33 -0.2 4,983,719 + BWI76_RS23875 0.59 -0.9 4,983,737 + BWI76_RS23875 0.64 +0.2 4,984,179 + BWI76_RS23880 0.25 -1.3 4,984,294 + BWI76_RS23880 0.36 -2.0
Or see this region's nucleotide sequence