Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20685

Experiment: D,L-Malic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20675 and BWI76_RS20680 are separated by 45 nucleotidesBWI76_RS20680 and BWI76_RS20685 overlap by 4 nucleotidesBWI76_RS20685 and BWI76_RS20690 are separated by 118 nucleotides BWI76_RS20675: BWI76_RS20675 - GMP synthase, at 4,325,683 to 4,326,390 _RS20675 BWI76_RS20680: BWI76_RS20680 - hypothetical protein, at 4,326,436 to 4,326,840 _RS20680 BWI76_RS20685: BWI76_RS20685 - cysteine synthase B, at 4,326,837 to 4,327,748 _RS20685 BWI76_RS20690: BWI76_RS20690 - sulfate ABC transporter ATP-binding protein, at 4,327,867 to 4,328,961 _RS20690 Position (kb) 4326 4327 4328Strain fitness (log2 ratio) -2 -1 0 1at 4325.864 kb on + strand, within BWI76_RS20675at 4325.904 kb on + strand, within BWI76_RS20675at 4326.249 kb on + strand, within BWI76_RS20675at 4326.249 kb on + strandat 4326.258 kb on - strand, within BWI76_RS20675at 4326.315 kb on + strand, within BWI76_RS20675at 4327.165 kb on - strand, within BWI76_RS20685at 4327.801 kb on - strandat 4327.822 kb on - strandat 4327.979 kb on + strand, within BWI76_RS20690at 4328.069 kb on + strand, within BWI76_RS20690at 4328.214 kb on - strand, within BWI76_RS20690at 4328.633 kb on + strand, within BWI76_RS20690

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Malic Acid (C)
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4,325,864 + BWI76_RS20675 0.26 +0.1
4,325,904 + BWI76_RS20675 0.31 +0.5
4,326,249 + BWI76_RS20675 0.80 -0.7
4,326,249 + -0.2
4,326,258 - BWI76_RS20675 0.81 +0.8
4,326,315 + BWI76_RS20675 0.89 -1.8
4,327,165 - BWI76_RS20685 0.36 +0.3
4,327,801 - -0.4
4,327,822 - +0.5
4,327,979 + BWI76_RS20690 0.10 -0.9
4,328,069 + BWI76_RS20690 0.18 -1.4
4,328,214 - BWI76_RS20690 0.32 -1.1
4,328,633 + BWI76_RS20690 0.70 -1.4

Or see this region's nucleotide sequence