Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20405

Experiment: D,L-Malic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20400 and BWI76_RS20405 are separated by 206 nucleotidesBWI76_RS20405 and BWI76_RS20410 are separated by 158 nucleotides BWI76_RS20400: BWI76_RS20400 - arabinose transporter, at 4,270,029 to 4,271,204 _RS20400 BWI76_RS20405: BWI76_RS20405 - beta-ketoacyl-[acyl-carrier-protein] synthase I, at 4,271,411 to 4,272,628 _RS20405 BWI76_RS20410: BWI76_RS20410 - bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC, at 4,272,787 to 4,274,775 _RS20410 Position (kb) 4271 4272 4273Strain fitness (log2 ratio) -1 0 1at 4270.442 kb on + strand, within BWI76_RS20400at 4270.450 kb on - strand, within BWI76_RS20400at 4270.935 kb on + strand, within BWI76_RS20400at 4271.116 kb on + strandat 4273.075 kb on - strand, within BWI76_RS20410at 4273.129 kb on - strand, within BWI76_RS20410at 4273.202 kb on + strand, within BWI76_RS20410at 4273.381 kb on + strand, within BWI76_RS20410

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Malic Acid (C)
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4,270,442 + BWI76_RS20400 0.35 +0.2
4,270,450 - BWI76_RS20400 0.36 -0.2
4,270,935 + BWI76_RS20400 0.77 +0.1
4,271,116 + -0.1
4,273,075 - BWI76_RS20410 0.14 +0.3
4,273,129 - BWI76_RS20410 0.17 -0.1
4,273,202 + BWI76_RS20410 0.21 +0.7
4,273,381 + BWI76_RS20410 0.30 +0.3

Or see this region's nucleotide sequence