Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18935

Experiment: D,L-Malic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18930 and BWI76_RS18935 overlap by 4 nucleotidesBWI76_RS18935 and BWI76_RS18940 overlap by 1 nucleotidesBWI76_RS18940 and BWI76_RS18945 overlap by 1 nucleotides BWI76_RS18930: BWI76_RS18930 - histidinol-phosphate transaminase, at 3,926,839 to 3,927,900 _RS18930 BWI76_RS18935: BWI76_RS18935 - bifunctional imidazole glycerol-phosphate dehydratase/histidinol phosphatase, at 3,927,897 to 3,928,964 _RS18935 BWI76_RS18940: BWI76_RS18940 - imidazole glycerol phosphate synthase subunit HisH, at 3,928,964 to 3,929,554 _RS18940 BWI76_RS18945: BWI76_RS18945 - 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase, at 3,929,554 to 3,930,291 _RS18945 Position (kb) 3927 3928 3929Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 3926.960 kb on + strand, within BWI76_RS18930at 3927.342 kb on + strand, within BWI76_RS18930at 3927.645 kb on - strand, within BWI76_RS18930at 3927.841 kb on + strandat 3928.350 kb on + strand, within BWI76_RS18935at 3928.358 kb on - strand, within BWI76_RS18935at 3928.547 kb on + strand, within BWI76_RS18935at 3928.788 kb on + strand, within BWI76_RS18935at 3928.813 kb on + strand, within BWI76_RS18935at 3928.820 kb on - strand, within BWI76_RS18935at 3928.839 kb on - strand, within BWI76_RS18935at 3929.066 kb on + strand, within BWI76_RS18940at 3929.157 kb on - strand, within BWI76_RS18940at 3929.368 kb on - strand, within BWI76_RS18940at 3929.441 kb on + strand, within BWI76_RS18940at 3929.476 kb on + strand, within BWI76_RS18940

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Malic Acid (C)
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3,926,960 + BWI76_RS18930 0.11 -3.5
3,927,342 + BWI76_RS18930 0.47 -5.2
3,927,645 - BWI76_RS18930 0.76 -6.8
3,927,841 + -5.3
3,928,350 + BWI76_RS18935 0.42 -3.9
3,928,358 - BWI76_RS18935 0.43 -4.5
3,928,547 + BWI76_RS18935 0.61 -2.1
3,928,788 + BWI76_RS18935 0.83 -6.3
3,928,813 + BWI76_RS18935 0.86 -5.2
3,928,820 - BWI76_RS18935 0.86 -3.1
3,928,839 - BWI76_RS18935 0.88 -6.2
3,929,066 + BWI76_RS18940 0.17 -4.3
3,929,157 - BWI76_RS18940 0.33 -2.5
3,929,368 - BWI76_RS18940 0.68 -4.6
3,929,441 + BWI76_RS18940 0.81 -4.2
3,929,476 + BWI76_RS18940 0.87 -3.5

Or see this region's nucleotide sequence