Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18305

Experiment: D,L-Malic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18295 and BWI76_RS18300 are separated by 30 nucleotidesBWI76_RS18300 and BWI76_RS18305 are separated by 66 nucleotidesBWI76_RS18305 and BWI76_RS18310 are separated by 190 nucleotidesBWI76_RS18310 and BWI76_RS18315 are separated by 13 nucleotidesBWI76_RS18315 and BWI76_RS18320 are separated by 38 nucleotidesBWI76_RS18320 and BWI76_RS18325 are separated by 151 nucleotides BWI76_RS18295: BWI76_RS18295 - tyrosine transporter TyrP, at 3,801,476 to 3,802,687 _RS18295 BWI76_RS18300: BWI76_RS18300 - hypothetical protein, at 3,802,718 to 3,803,386 _RS18300 BWI76_RS18305: BWI76_RS18305 - dihydrodipicolinate synthase family protein, at 3,803,453 to 3,804,340 _RS18305 BWI76_RS18310: BWI76_RS18310 - tRNA-Leu, at 3,804,531 to 3,804,617 _RS18310 BWI76_RS18315: BWI76_RS18315 - tRNA-Cys, at 3,804,631 to 3,804,704 _RS18315 BWI76_RS18320: BWI76_RS18320 - tRNA-Gly, at 3,804,743 to 3,804,818 _RS18320 BWI76_RS18325: BWI76_RS18325 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,804,970 to 3,805,518 _RS18325 Position (kb) 3803 3804 3805Strain fitness (log2 ratio) -1 0 1at 3802.481 kb on + strand, within BWI76_RS18295at 3802.672 kb on - strandat 3802.788 kb on - strand, within BWI76_RS18300at 3802.839 kb on + strand, within BWI76_RS18300at 3803.016 kb on - strand, within BWI76_RS18300at 3803.249 kb on - strand, within BWI76_RS18300at 3803.975 kb on - strand, within BWI76_RS18305at 3804.128 kb on + strand, within BWI76_RS18305

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Malic Acid (C)
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3,802,481 + BWI76_RS18295 0.83 +0.3
3,802,672 - -0.2
3,802,788 - BWI76_RS18300 0.10 +1.2
3,802,839 + BWI76_RS18300 0.18 -0.8
3,803,016 - BWI76_RS18300 0.45 -0.0
3,803,249 - BWI76_RS18300 0.79 -0.1
3,803,975 - BWI76_RS18305 0.59 +0.4
3,804,128 + BWI76_RS18305 0.76 -0.1

Or see this region's nucleotide sequence