Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05685

Experiment: D,L-Malic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05680 and BWI76_RS05685 are separated by 251 nucleotidesBWI76_RS05685 and BWI76_RS05690 are separated by 143 nucleotides BWI76_RS05680: BWI76_RS05680 - LacI family transcriptional regulator, at 1,194,032 to 1,195,063 _RS05680 BWI76_RS05685: BWI76_RS05685 - dihydrodipicolinate synthase family protein, at 1,195,315 to 1,196,244 _RS05685 BWI76_RS05690: BWI76_RS05690 - fumarylacetoacetate (FAA) hydrolase, at 1,196,388 to 1,197,236 _RS05690 Position (kb) 1195 1196 1197Strain fitness (log2 ratio) -3 -2 -1 0 1at 1194.323 kb on - strand, within BWI76_RS05680at 1194.407 kb on + strand, within BWI76_RS05680at 1194.473 kb on + strand, within BWI76_RS05680at 1194.530 kb on - strand, within BWI76_RS05680at 1194.621 kb on - strand, within BWI76_RS05680at 1195.023 kb on + strandat 1195.441 kb on + strand, within BWI76_RS05685at 1195.681 kb on + strand, within BWI76_RS05685at 1195.691 kb on + strand, within BWI76_RS05685at 1195.740 kb on + strand, within BWI76_RS05685at 1195.887 kb on - strand, within BWI76_RS05685at 1195.920 kb on + strand, within BWI76_RS05685at 1195.926 kb on - strand, within BWI76_RS05685at 1195.939 kb on - strand, within BWI76_RS05685at 1195.945 kb on - strand, within BWI76_RS05685at 1196.021 kb on + strand, within BWI76_RS05685at 1196.030 kb on - strand, within BWI76_RS05685at 1196.133 kb on + strand, within BWI76_RS05685at 1196.146 kb on - strand, within BWI76_RS05685at 1196.219 kb on + strandat 1196.232 kb on - strandat 1196.239 kb on + strandat 1196.243 kb on + strandat 1196.293 kb on - strandat 1196.363 kb on + strandat 1196.364 kb on - strandat 1196.368 kb on + strandat 1196.371 kb on - strandat 1196.374 kb on + strandat 1196.394 kb on + strandat 1196.466 kb on - strandat 1196.509 kb on + strand, within BWI76_RS05690at 1196.509 kb on + strand, within BWI76_RS05690at 1196.510 kb on + strand, within BWI76_RS05690at 1196.517 kb on - strand, within BWI76_RS05690at 1196.517 kb on - strand, within BWI76_RS05690at 1196.517 kb on - strand, within BWI76_RS05690at 1196.517 kb on - strand, within BWI76_RS05690at 1196.519 kb on + strand, within BWI76_RS05690at 1196.527 kb on + strand, within BWI76_RS05690at 1196.527 kb on - strand, within BWI76_RS05690at 1196.527 kb on - strand, within BWI76_RS05690at 1196.531 kb on + strand, within BWI76_RS05690at 1196.662 kb on - strand, within BWI76_RS05690at 1196.723 kb on + strand, within BWI76_RS05690at 1196.760 kb on + strand, within BWI76_RS05690at 1196.800 kb on - strand, within BWI76_RS05690at 1196.808 kb on - strand, within BWI76_RS05690at 1196.826 kb on + strand, within BWI76_RS05690at 1197.016 kb on - strand, within BWI76_RS05690at 1197.077 kb on + strand, within BWI76_RS05690at 1197.205 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Malic Acid (C)
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1,194,323 - BWI76_RS05680 0.28 -1.3
1,194,407 + BWI76_RS05680 0.36 -1.4
1,194,473 + BWI76_RS05680 0.43 -2.2
1,194,530 - BWI76_RS05680 0.48 -1.2
1,194,621 - BWI76_RS05680 0.57 -2.7
1,195,023 + -3.0
1,195,441 + BWI76_RS05685 0.14 +1.1
1,195,681 + BWI76_RS05685 0.39 -0.5
1,195,691 + BWI76_RS05685 0.40 +1.6
1,195,740 + BWI76_RS05685 0.46 -0.2
1,195,887 - BWI76_RS05685 0.62 -0.0
1,195,920 + BWI76_RS05685 0.65 -0.0
1,195,926 - BWI76_RS05685 0.66 -1.5
1,195,939 - BWI76_RS05685 0.67 +0.8
1,195,945 - BWI76_RS05685 0.68 +0.9
1,196,021 + BWI76_RS05685 0.76 +0.3
1,196,030 - BWI76_RS05685 0.77 +0.2
1,196,133 + BWI76_RS05685 0.88 -0.1
1,196,146 - BWI76_RS05685 0.89 -0.2
1,196,219 + -0.8
1,196,232 - +1.5
1,196,239 + -3.0
1,196,243 + +0.2
1,196,293 - +0.8
1,196,363 + +0.4
1,196,364 - +0.2
1,196,368 + +0.0
1,196,371 - +0.3
1,196,374 + -0.4
1,196,394 + -0.2
1,196,466 - +0.1
1,196,509 + BWI76_RS05690 0.14 +0.6
1,196,509 + BWI76_RS05690 0.14 -0.3
1,196,510 + BWI76_RS05690 0.14 +1.3
1,196,517 - BWI76_RS05690 0.15 -0.3
1,196,517 - BWI76_RS05690 0.15 -0.6
1,196,517 - BWI76_RS05690 0.15 -0.1
1,196,517 - BWI76_RS05690 0.15 -0.3
1,196,519 + BWI76_RS05690 0.15 +0.1
1,196,527 + BWI76_RS05690 0.16 +0.6
1,196,527 - BWI76_RS05690 0.16 -0.1
1,196,527 - BWI76_RS05690 0.16 +0.3
1,196,531 + BWI76_RS05690 0.17 -0.3
1,196,662 - BWI76_RS05690 0.32 +0.3
1,196,723 + BWI76_RS05690 0.39 -0.7
1,196,760 + BWI76_RS05690 0.44 +0.4
1,196,800 - BWI76_RS05690 0.49 -0.1
1,196,808 - BWI76_RS05690 0.49 +1.0
1,196,826 + BWI76_RS05690 0.52 +0.2
1,197,016 - BWI76_RS05690 0.74 +0.5
1,197,077 + BWI76_RS05690 0.81 +0.8
1,197,205 - +1.1

Or see this region's nucleotide sequence