Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21365 and AO356_21370 are separated by 28 nucleotides AO356_21370 and AO356_21375 are separated by 85 nucleotides AO356_21375 and AO356_21380 are separated by 382 nucleotides
AO356_21365: AO356_21365 - TetR family transcriptional regulator, at 4,789,615 to 4,790,154
_21365
AO356_21370: AO356_21370 - hydrolase, at 4,790,183 to 4,791,016
_21370
AO356_21375: AO356_21375 - 4-hydroxybenzoyl-CoA thioesterase, at 4,791,102 to 4,791,551
_21375
AO356_21380: AO356_21380 - tRNA-dihydrouridine synthase C, at 4,791,934 to 4,792,893
_21380
Position (kb) 4791
4792 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4790.202 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.206 kb on + strand at 4790.207 kb on - strand at 4790.207 kb on - strand at 4790.207 kb on - strand at 4790.207 kb on - strand at 4790.207 kb on - strand at 4790.335 kb on + strand, within AO356_21370 at 4790.336 kb on - strand, within AO356_21370 at 4790.336 kb on - strand, within AO356_21370 at 4790.462 kb on + strand, within AO356_21370 at 4790.802 kb on - strand, within AO356_21370 at 4790.970 kb on + strand at 4791.055 kb on + strand at 4791.055 kb on + strand at 4791.055 kb on + strand at 4791.055 kb on + strand at 4791.056 kb on - strand at 4791.190 kb on + strand, within AO356_21375 at 4791.192 kb on + strand, within AO356_21375 at 4791.192 kb on + strand, within AO356_21375 at 4791.192 kb on + strand, within AO356_21375 at 4791.192 kb on + strand, within AO356_21375 at 4791.193 kb on - strand, within AO356_21375 at 4791.193 kb on - strand, within AO356_21375 at 4791.193 kb on - strand, within AO356_21375 at 4791.193 kb on - strand, within AO356_21375 at 4791.193 kb on - strand, within AO356_21375 at 4791.259 kb on - strand, within AO356_21375 at 4791.306 kb on + strand, within AO356_21375 at 4791.321 kb on + strand, within AO356_21375 at 4791.322 kb on - strand, within AO356_21375 at 4791.603 kb on + strand at 4791.603 kb on + strand at 4791.603 kb on + strand at 4791.604 kb on - strand at 4791.604 kb on - strand at 4791.604 kb on - strand at 4791.604 kb on - strand at 4791.789 kb on - strand at 4791.797 kb on + strand at 4792.066 kb on + strand, within AO356_21380 at 4792.067 kb on - strand, within AO356_21380 at 4792.067 kb on - strand, within AO356_21380 at 4792.074 kb on + strand, within AO356_21380 at 4792.074 kb on + strand, within AO356_21380 at 4792.459 kb on + strand, within AO356_21380 at 4792.459 kb on + strand, within AO356_21380 at 4792.459 kb on + strand, within AO356_21380 at 4792.459 kb on + strand, within AO356_21380 at 4792.460 kb on - strand, within AO356_21380
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 4,790,202 + +0.3 4,790,206 + +0.6 4,790,206 + -3.2 4,790,206 + +0.6 4,790,206 + +0.7 4,790,206 + +0.7 4,790,206 + +0.3 4,790,206 + -0.8 4,790,206 + +0.2 4,790,206 + +0.2 4,790,206 + +0.4 4,790,207 - +0.4 4,790,207 - -0.2 4,790,207 - +0.1 4,790,207 - -0.4 4,790,207 - +0.1 4,790,335 + AO356_21370 0.18 -0.2 4,790,336 - AO356_21370 0.18 +0.4 4,790,336 - AO356_21370 0.18 -1.0 4,790,462 + AO356_21370 0.33 -0.3 4,790,802 - AO356_21370 0.74 +0.5 4,790,970 + +0.0 4,791,055 + -0.5 4,791,055 + +0.1 4,791,055 + -0.3 4,791,055 + -0.1 4,791,056 - +0.3 4,791,190 + AO356_21375 0.20 -0.8 4,791,192 + AO356_21375 0.20 +0.1 4,791,192 + AO356_21375 0.20 -1.9 4,791,192 + AO356_21375 0.20 -1.4 4,791,192 + AO356_21375 0.20 -0.1 4,791,193 - AO356_21375 0.20 -0.1 4,791,193 - AO356_21375 0.20 -0.1 4,791,193 - AO356_21375 0.20 -0.1 4,791,193 - AO356_21375 0.20 +1.1 4,791,193 - AO356_21375 0.20 -0.7 4,791,259 - AO356_21375 0.35 -0.4 4,791,306 + AO356_21375 0.45 -0.4 4,791,321 + AO356_21375 0.49 +0.1 4,791,322 - AO356_21375 0.49 +0.2 4,791,603 + +0.4 4,791,603 + +0.0 4,791,603 + +0.9 4,791,604 - -0.0 4,791,604 - +0.5 4,791,604 - -0.4 4,791,604 - +0.8 4,791,789 - +0.2 4,791,797 + +1.2 4,792,066 + AO356_21380 0.14 -0.2 4,792,067 - AO356_21380 0.14 +0.5 4,792,067 - AO356_21380 0.14 +0.9 4,792,074 + AO356_21380 0.15 +0.4 4,792,074 + AO356_21380 0.15 -0.5 4,792,459 + AO356_21380 0.55 +0.9 4,792,459 + AO356_21380 0.55 +0.1 4,792,459 + AO356_21380 0.55 +0.5 4,792,459 + AO356_21380 0.55 +0.5 4,792,460 - AO356_21380 0.55 +0.3
Or see this region's nucleotide sequence