Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_08975 and AO356_08980 are separated by 84 nucleotides AO356_08980 and AO356_08985 are separated by 3 nucleotides AO356_08985 and AO356_08990 are separated by 1 nucleotides AO356_08990 and AO356_08995 are separated by 81 nucleotides AO356_08995 and AO356_09000 overlap by 4 nucleotides
AO356_08975: AO356_08975 - diguanylate cyclase, at 2,028,749 to 2,031,442
_08975
AO356_08980: AO356_08980 - peptide methionine sulfoxide reductase, at 2,031,527 to 2,032,174
_08980
AO356_08985: AO356_08985 - hypothetical protein, at 2,032,178 to 2,032,378
_08985
AO356_08990: AO356_08990 - lactate dehydrogenase, at 2,032,380 to 2,033,219
_08990
AO356_08995: AO356_08995 - hypothetical protein, at 2,033,301 to 2,034,032
_08995
AO356_09000: AO356_09000 - type IV secretion protein Rhs, at 2,034,029 to 2,036,086
_09000
Position (kb)
2032
2033
2034 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2031.451 kb on + strand at 2031.452 kb on - strand at 2031.452 kb on - strand at 2031.454 kb on - strand at 2031.454 kb on - strand at 2031.454 kb on - strand at 2031.490 kb on + strand at 2031.491 kb on - strand at 2031.491 kb on - strand at 2031.525 kb on + strand at 2031.526 kb on - strand at 2031.556 kb on + strand at 2031.556 kb on + strand at 2031.557 kb on - strand at 2031.572 kb on - strand at 2031.762 kb on - strand, within AO356_08980 at 2031.791 kb on + strand, within AO356_08980 at 2031.791 kb on + strand, within AO356_08980 at 2031.791 kb on + strand, within AO356_08980 at 2031.792 kb on - strand, within AO356_08980 at 2031.792 kb on - strand, within AO356_08980 at 2031.845 kb on + strand, within AO356_08980 at 2031.846 kb on - strand, within AO356_08980 at 2031.866 kb on + strand, within AO356_08980 at 2031.866 kb on + strand, within AO356_08980 at 2031.867 kb on - strand, within AO356_08980 at 2031.867 kb on - strand, within AO356_08980 at 2031.962 kb on + strand, within AO356_08980 at 2031.962 kb on + strand, within AO356_08980 at 2031.962 kb on + strand, within AO356_08980 at 2031.962 kb on + strand, within AO356_08980 at 2031.962 kb on + strand, within AO356_08980 at 2031.962 kb on + strand, within AO356_08980 at 2031.963 kb on - strand, within AO356_08980 at 2031.963 kb on - strand, within AO356_08980 at 2031.963 kb on - strand, within AO356_08980 at 2031.963 kb on - strand, within AO356_08980 at 2031.963 kb on - strand, within AO356_08980 at 2031.963 kb on - strand, within AO356_08980 at 2032.082 kb on + strand, within AO356_08980 at 2032.097 kb on + strand, within AO356_08980 at 2032.097 kb on + strand, within AO356_08980 at 2032.097 kb on + strand, within AO356_08980 at 2032.097 kb on + strand, within AO356_08980 at 2032.098 kb on - strand, within AO356_08980 at 2032.098 kb on - strand, within AO356_08980 at 2032.109 kb on + strand, within AO356_08980 at 2032.110 kb on - strand at 2032.110 kb on - strand at 2032.110 kb on - strand at 2032.110 kb on - strand at 2032.179 kb on + strand at 2032.179 kb on + strand at 2032.179 kb on + strand at 2032.179 kb on + strand at 2032.179 kb on + strand at 2032.179 kb on + strand at 2032.180 kb on - strand at 2032.257 kb on + strand, within AO356_08985 at 2032.600 kb on + strand, within AO356_08990 at 2032.936 kb on + strand, within AO356_08990 at 2032.937 kb on - strand, within AO356_08990 at 2032.937 kb on - strand, within AO356_08990 at 2032.981 kb on + strand, within AO356_08990 at 2032.982 kb on - strand, within AO356_08990 at 2033.041 kb on + strand, within AO356_08990 at 2033.041 kb on + strand, within AO356_08990 at 2033.041 kb on + strand, within AO356_08990 at 2033.041 kb on + strand, within AO356_08990 at 2033.042 kb on - strand, within AO356_08990 at 2033.080 kb on + strand, within AO356_08990 at 2033.080 kb on + strand, within AO356_08990 at 2033.080 kb on + strand, within AO356_08990 at 2033.081 kb on - strand, within AO356_08990 at 2033.081 kb on - strand, within AO356_08990 at 2033.110 kb on + strand, within AO356_08990 at 2033.213 kb on - strand at 2033.247 kb on + strand at 2033.248 kb on - strand at 2033.248 kb on - strand at 2033.323 kb on + strand at 2033.323 kb on + strand at 2033.324 kb on - strand at 2033.324 kb on - strand at 2033.324 kb on - strand at 2033.324 kb on - strand at 2033.324 kb on - strand at 2033.419 kb on + strand, within AO356_08995 at 2033.562 kb on + strand, within AO356_08995 at 2033.563 kb on - strand, within AO356_08995 at 2033.838 kb on + strand, within AO356_08995 at 2033.838 kb on + strand, within AO356_08995 at 2033.838 kb on + strand, within AO356_08995 at 2033.838 kb on + strand, within AO356_08995 at 2033.838 kb on + strand, within AO356_08995 at 2033.838 kb on + strand, within AO356_08995 at 2033.839 kb on - strand, within AO356_08995 at 2033.839 kb on - strand, within AO356_08995 at 2033.842 kb on + strand, within AO356_08995 at 2033.842 kb on + strand, within AO356_08995 at 2033.842 kb on + strand, within AO356_08995 at 2033.842 kb on + strand, within AO356_08995 at 2033.842 kb on + strand, within AO356_08995 at 2033.842 kb on + strand, within AO356_08995 at 2033.842 kb on + strand, within AO356_08995 at 2033.843 kb on - strand, within AO356_08995 at 2033.843 kb on - strand, within AO356_08995
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 2,031,451 + +0.1 2,031,452 - -0.9 2,031,452 - -1.2 2,031,454 - -0.0 2,031,454 - +0.1 2,031,454 - +0.9 2,031,490 + -0.6 2,031,491 - -0.5 2,031,491 - +0.7 2,031,525 + -0.0 2,031,526 - +0.1 2,031,556 + +0.1 2,031,556 + +0.0 2,031,557 - +0.1 2,031,572 - -0.8 2,031,762 - AO356_08980 0.36 +0.3 2,031,791 + AO356_08980 0.41 -0.8 2,031,791 + AO356_08980 0.41 +0.6 2,031,791 + AO356_08980 0.41 +0.3 2,031,792 - AO356_08980 0.41 +0.9 2,031,792 - AO356_08980 0.41 -0.8 2,031,845 + AO356_08980 0.49 -0.3 2,031,846 - AO356_08980 0.49 -0.4 2,031,866 + AO356_08980 0.52 +0.1 2,031,866 + AO356_08980 0.52 -1.1 2,031,867 - AO356_08980 0.52 +0.4 2,031,867 - AO356_08980 0.52 +0.5 2,031,962 + AO356_08980 0.67 -0.4 2,031,962 + AO356_08980 0.67 +0.1 2,031,962 + AO356_08980 0.67 +0.7 2,031,962 + AO356_08980 0.67 +0.7 2,031,962 + AO356_08980 0.67 -0.2 2,031,962 + AO356_08980 0.67 -0.5 2,031,963 - AO356_08980 0.67 +0.6 2,031,963 - AO356_08980 0.67 +1.5 2,031,963 - AO356_08980 0.67 +0.1 2,031,963 - AO356_08980 0.67 +0.5 2,031,963 - AO356_08980 0.67 +0.5 2,031,963 - AO356_08980 0.67 +0.1 2,032,082 + AO356_08980 0.86 +0.6 2,032,097 + AO356_08980 0.88 +0.3 2,032,097 + AO356_08980 0.88 -0.0 2,032,097 + AO356_08980 0.88 +0.8 2,032,097 + AO356_08980 0.88 -0.2 2,032,098 - AO356_08980 0.88 +0.9 2,032,098 - AO356_08980 0.88 -0.1 2,032,109 + AO356_08980 0.90 -0.2 2,032,110 - -0.0 2,032,110 - +0.4 2,032,110 - -0.1 2,032,110 - +0.5 2,032,179 + -0.4 2,032,179 + +0.7 2,032,179 + -0.0 2,032,179 + +0.5 2,032,179 + -3.2 2,032,179 + -0.4 2,032,180 - +0.3 2,032,257 + AO356_08985 0.39 +0.3 2,032,600 + AO356_08990 0.26 +1.8 2,032,936 + AO356_08990 0.66 -0.8 2,032,937 - AO356_08990 0.66 +0.7 2,032,937 - AO356_08990 0.66 +1.4 2,032,981 + AO356_08990 0.72 +2.3 2,032,982 - AO356_08990 0.72 +0.4 2,033,041 + AO356_08990 0.79 -0.3 2,033,041 + AO356_08990 0.79 +0.5 2,033,041 + AO356_08990 0.79 +0.9 2,033,041 + AO356_08990 0.79 +1.1 2,033,042 - AO356_08990 0.79 -0.6 2,033,080 + AO356_08990 0.83 -0.8 2,033,080 + AO356_08990 0.83 -0.1 2,033,080 + AO356_08990 0.83 +0.1 2,033,081 - AO356_08990 0.83 -0.2 2,033,081 - AO356_08990 0.83 -1.2 2,033,110 + AO356_08990 0.87 +0.7 2,033,213 - -0.8 2,033,247 + -0.4 2,033,248 - -0.6 2,033,248 - +1.2 2,033,323 + -2.0 2,033,323 + +0.3 2,033,324 - +0.3 2,033,324 - +0.2 2,033,324 - -0.8 2,033,324 - -0.5 2,033,324 - +0.1 2,033,419 + AO356_08995 0.16 +0.7 2,033,562 + AO356_08995 0.36 +1.3 2,033,563 - AO356_08995 0.36 -0.6 2,033,838 + AO356_08995 0.73 +0.7 2,033,838 + AO356_08995 0.73 +2.2 2,033,838 + AO356_08995 0.73 +1.0 2,033,838 + AO356_08995 0.73 +0.1 2,033,838 + AO356_08995 0.73 -0.6 2,033,838 + AO356_08995 0.73 -0.4 2,033,839 - AO356_08995 0.73 -0.1 2,033,839 - AO356_08995 0.73 +1.1 2,033,842 + AO356_08995 0.74 -1.1 2,033,842 + AO356_08995 0.74 -1.7 2,033,842 + AO356_08995 0.74 +1.1 2,033,842 + AO356_08995 0.74 -0.3 2,033,842 + AO356_08995 0.74 +0.2 2,033,842 + AO356_08995 0.74 +1.3 2,033,842 + AO356_08995 0.74 -0.8 2,033,843 - AO356_08995 0.74 +0.9 2,033,843 - AO356_08995 0.74 +0.2
Or see this region's nucleotide sequence