Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02235 and AO356_02240 are separated by 94 nucleotides AO356_02240 and AO356_02245 overlap by 4 nucleotides AO356_02245 and AO356_02250 are separated by 42 nucleotides
AO356_02235: AO356_02235 - cell division protein FtsX, at 479,186 to 480,436
_02235
AO356_02240: AO356_02240 - pilus assembly protein PilZ, at 480,531 to 481,127
_02240
AO356_02245: AO356_02245 - glycerophosphodiester phosphodiesterase, at 481,124 to 481,846
_02245
AO356_02250: AO356_02250 - pyridine nucleotide-disulfide oxidoreductase, at 481,889 to 483,283
_02250
Position (kb) 480
481
482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 480.497 kb on - strand at 480.497 kb on - strand at 480.497 kb on - strand at 480.497 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.565 kb on - strand at 480.750 kb on + strand, within AO356_02240 at 480.917 kb on - strand, within AO356_02240 at 481.047 kb on + strand, within AO356_02240 at 481.048 kb on - strand, within AO356_02240 at 481.106 kb on + strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.112 kb on + strand at 481.136 kb on + strand at 481.136 kb on + strand at 481.136 kb on + strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.212 kb on + strand, within AO356_02245 at 481.213 kb on - strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.344 kb on - strand, within AO356_02245 at 481.344 kb on - strand, within AO356_02245 at 481.613 kb on + strand, within AO356_02245 at 481.613 kb on + strand, within AO356_02245 at 481.649 kb on + strand, within AO356_02245 at 481.650 kb on - strand, within AO356_02245 at 481.801 kb on + strand at 481.801 kb on + strand at 481.802 kb on - strand at 481.914 kb on + strand at 481.951 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 480,497 - +2.4 480,497 - +1.0 480,497 - +0.2 480,497 - -0.4 480,542 - +0.2 480,542 - -0.1 480,542 - +1.5 480,542 - +1.2 480,565 - +0.4 480,750 + AO356_02240 0.37 -0.7 480,917 - AO356_02240 0.65 +0.5 481,047 + AO356_02240 0.86 +0.1 481,048 - AO356_02240 0.87 -0.1 481,106 + -0.5 481,107 - +0.6 481,107 - +1.0 481,107 - -1.1 481,107 - +0.1 481,112 + +0.1 481,136 + +0.1 481,136 + +0.1 481,136 + -1.1 481,137 - +0.9 481,137 - -0.2 481,137 - +0.8 481,137 - +0.6 481,137 - -0.2 481,212 + AO356_02245 0.12 +0.8 481,213 - AO356_02245 0.12 -0.2 481,343 + AO356_02245 0.30 -0.9 481,343 + AO356_02245 0.30 +0.8 481,343 + AO356_02245 0.30 +0.0 481,343 + AO356_02245 0.30 +0.4 481,344 - AO356_02245 0.30 +0.4 481,344 - AO356_02245 0.30 +1.1 481,613 + AO356_02245 0.68 -0.4 481,613 + AO356_02245 0.68 -1.3 481,649 + AO356_02245 0.73 +0.4 481,650 - AO356_02245 0.73 -0.4 481,801 + -1.7 481,801 + -0.1 481,802 - -0.8 481,914 + -2.9 481,951 - -3.2
Or see this region's nucleotide sequence