Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02010 and AO356_02015 are separated by 96 nucleotides AO356_02015 and AO356_02020 overlap by 4 nucleotides AO356_02020 and AO356_02025 overlap by 6 nucleotides AO356_02025 and AO356_02030 are separated by 478 nucleotides
AO356_02010: AO356_02010 - peptidase, at 442,872 to 443,315
_02010
AO356_02015: AO356_02015 - phage tail protein, at 443,412 to 443,888
_02015
AO356_02020: AO356_02020 - phage tail protein, at 443,885 to 444,349
_02020
AO356_02025: AO356_02025 - hypothetical protein, at 444,344 to 444,790
_02025
AO356_02030: AO356_02030 - baseplate assembly protein, at 445,269 to 445,841
_02030
Position (kb) 443
444
445 Strain fitness (log2 ratio) -1
0
1
2 at 443.389 kb on + strand at 443.390 kb on - strand at 443.390 kb on - strand at 443.548 kb on - strand, within AO356_02015 at 443.560 kb on + strand, within AO356_02015 at 443.561 kb on - strand, within AO356_02015 at 443.581 kb on - strand, within AO356_02015 at 443.728 kb on + strand, within AO356_02015 at 443.728 kb on + strand, within AO356_02015 at 443.728 kb on + strand, within AO356_02015 at 443.728 kb on + strand, within AO356_02015 at 443.729 kb on - strand, within AO356_02015 at 443.729 kb on - strand, within AO356_02015 at 443.729 kb on - strand, within AO356_02015 at 443.780 kb on + strand, within AO356_02015 at 443.807 kb on + strand, within AO356_02015 at 443.807 kb on + strand, within AO356_02015 at 443.807 kb on + strand, within AO356_02015 at 443.808 kb on - strand, within AO356_02015 at 443.808 kb on - strand, within AO356_02015 at 443.808 kb on - strand, within AO356_02015 at 443.808 kb on - strand, within AO356_02015 at 443.808 kb on - strand, within AO356_02015 at 443.808 kb on - strand, within AO356_02015 at 443.808 kb on - strand, within AO356_02015 at 443.843 kb on - strand at 444.044 kb on + strand, within AO356_02020 at 444.044 kb on + strand, within AO356_02020 at 444.044 kb on + strand, within AO356_02020 at 444.045 kb on - strand, within AO356_02020 at 444.045 kb on - strand, within AO356_02020 at 444.164 kb on - strand, within AO356_02020 at 444.166 kb on + strand, within AO356_02020 at 444.216 kb on - strand, within AO356_02020 at 444.277 kb on - strand, within AO356_02020 at 444.277 kb on - strand, within AO356_02020 at 444.277 kb on - strand, within AO356_02020 at 444.301 kb on - strand, within AO356_02020 at 444.358 kb on + strand at 444.405 kb on + strand, within AO356_02025 at 444.669 kb on - strand, within AO356_02025 at 444.724 kb on - strand, within AO356_02025 at 444.734 kb on - strand, within AO356_02025 at 444.749 kb on - strand at 444.869 kb on + strand at 444.965 kb on - strand at 445.112 kb on + strand at 445.113 kb on - strand at 445.137 kb on + strand at 445.218 kb on + strand at 445.218 kb on + strand at 445.218 kb on + strand at 445.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 443,389 + -0.8 443,390 - -1.0 443,390 - +0.6 443,548 - AO356_02015 0.29 +0.2 443,560 + AO356_02015 0.31 -0.3 443,561 - AO356_02015 0.31 -0.6 443,581 - AO356_02015 0.35 +1.8 443,728 + AO356_02015 0.66 -0.8 443,728 + AO356_02015 0.66 +0.9 443,728 + AO356_02015 0.66 -0.6 443,728 + AO356_02015 0.66 +0.9 443,729 - AO356_02015 0.66 -0.1 443,729 - AO356_02015 0.66 +0.4 443,729 - AO356_02015 0.66 +0.2 443,780 + AO356_02015 0.77 -0.2 443,807 + AO356_02015 0.83 -1.4 443,807 + AO356_02015 0.83 +0.8 443,807 + AO356_02015 0.83 +0.3 443,808 - AO356_02015 0.83 -0.6 443,808 - AO356_02015 0.83 -0.4 443,808 - AO356_02015 0.83 -0.1 443,808 - AO356_02015 0.83 +0.4 443,808 - AO356_02015 0.83 -1.5 443,808 - AO356_02015 0.83 +0.5 443,808 - AO356_02015 0.83 +0.3 443,843 - -1.1 444,044 + AO356_02020 0.34 -0.2 444,044 + AO356_02020 0.34 +0.3 444,044 + AO356_02020 0.34 +1.4 444,045 - AO356_02020 0.34 -0.0 444,045 - AO356_02020 0.34 -0.9 444,164 - AO356_02020 0.60 -0.4 444,166 + AO356_02020 0.60 +1.9 444,216 - AO356_02020 0.71 +0.5 444,277 - AO356_02020 0.84 +0.2 444,277 - AO356_02020 0.84 -0.4 444,277 - AO356_02020 0.84 +2.4 444,301 - AO356_02020 0.89 -0.2 444,358 + -0.4 444,405 + AO356_02025 0.14 -0.4 444,669 - AO356_02025 0.73 -0.9 444,724 - AO356_02025 0.85 +0.1 444,734 - AO356_02025 0.87 -0.7 444,749 - +0.9 444,869 + -0.0 444,965 - -0.6 445,112 + -0.2 445,113 - -1.1 445,137 + +0.3 445,218 + +0.1 445,218 + +0.1 445,218 + -0.5 445,280 - -0.3
Or see this region's nucleotide sequence