Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3597 and Shew_3598 are separated by 169 nucleotides Shew_3598 and Shew_3599 are separated by 99 nucleotides
Shew_3597: Shew_3597 - inner membrane protein YjeH (RefSeq), at 4,294,383 to 4,295,642
_3597
Shew_3598: Shew_3598 - AsnC family transcriptional regulator (RefSeq), at 4,295,812 to 4,296,240
_3598
Shew_3599: Shew_3599 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase (RefSeq), at 4,296,340 to 4,298,043
_3599
Position (kb)
4295
4296
4297 Strain fitness (log2 ratio)
-1
0
1
2 at 4294.847 kb on + strand, within Shew_3597 at 4294.914 kb on - strand, within Shew_3597 at 4295.120 kb on + strand, within Shew_3597 at 4295.143 kb on - strand, within Shew_3597 at 4295.149 kb on + strand, within Shew_3597 at 4295.519 kb on - strand at 4295.827 kb on - strand at 4295.957 kb on + strand, within Shew_3598 at 4296.416 kb on - strand at 4296.533 kb on + strand, within Shew_3599 at 4296.551 kb on - strand, within Shew_3599 at 4296.619 kb on + strand, within Shew_3599 at 4296.827 kb on - strand, within Shew_3599 at 4296.845 kb on - strand, within Shew_3599 at 4296.895 kb on + strand, within Shew_3599 at 4296.971 kb on - strand, within Shew_3599 at 4297.184 kb on - strand, within Shew_3599 at 4297.215 kb on - strand, within Shew_3599
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,294,847 + Shew_3597 0.37 -0.6 4,294,914 - Shew_3597 0.42 -0.1 4,295,120 + Shew_3597 0.58 -0.3 4,295,143 - Shew_3597 0.60 +0.9 4,295,149 + Shew_3597 0.61 +0.9 4,295,519 - +0.1 4,295,827 - -0.6 4,295,957 + Shew_3598 0.34 -0.8 4,296,416 - -0.7 4,296,533 + Shew_3599 0.11 -0.0 4,296,551 - Shew_3599 0.12 -0.1 4,296,619 + Shew_3599 0.16 +1.3 4,296,827 - Shew_3599 0.29 +1.9 4,296,845 - Shew_3599 0.30 +0.5 4,296,895 + Shew_3599 0.33 +0.4 4,296,971 - Shew_3599 0.37 -1.5 4,297,184 - Shew_3599 0.50 +0.2 4,297,215 - Shew_3599 0.51 -0.1
Or see this region's nucleotide sequence