Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3520 and Shew_3521 overlap by 4 nucleotides Shew_3521 and Shew_3522 overlap by 10 nucleotides Shew_3522 and Shew_3523 are separated by 32 nucleotides
Shew_3520: Shew_3520 - hypothetical protein (RefSeq), at 4,205,923 to 4,208,448
_3520
Shew_3521: Shew_3521 - tryptophan halogenase (RefSeq), at 4,208,445 to 4,209,719
_3521
Shew_3522: Shew_3522 - hypothetical protein (RefSeq), at 4,209,710 to 4,210,282
_3522
Shew_3523: Shew_3523 - 3-oxoacyl-(acyl carrier protein) synthase I (RefSeq), at 4,210,315 to 4,211,520
_3523
Position (kb) 4208
4209
4210 Strain fitness (log2 ratio) -8
-7
-6
-5
-4
-3
-2
-1
0
1 at 4207.549 kb on - strand, within Shew_3520 at 4208.300 kb on - strand at 4208.382 kb on + strand at 4208.392 kb on + strand at 4208.392 kb on + strand at 4208.392 kb on + strand at 4208.400 kb on - strand at 4208.615 kb on + strand, within Shew_3521 at 4208.677 kb on - strand, within Shew_3521 at 4208.847 kb on - strand, within Shew_3521 at 4208.873 kb on + strand, within Shew_3521 at 4208.879 kb on + strand, within Shew_3521 at 4209.127 kb on + strand, within Shew_3521 at 4209.411 kb on + strand, within Shew_3521 at 4209.615 kb on - strand at 4209.638 kb on - strand at 4209.809 kb on + strand, within Shew_3522 at 4209.876 kb on + strand, within Shew_3522 at 4210.277 kb on + strand at 4210.477 kb on - strand, within Shew_3523 at 4210.604 kb on + strand, within Shew_3523 at 4210.716 kb on + strand, within Shew_3523
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,207,549 - Shew_3520 0.64 -6.3 4,208,300 - -7.9 4,208,382 + -4.6 4,208,392 + -5.1 4,208,392 + -8.5 4,208,392 + -5.9 4,208,400 - -6.0 4,208,615 + Shew_3521 0.13 -3.5 4,208,677 - Shew_3521 0.18 -4.2 4,208,847 - Shew_3521 0.32 -8.0 4,208,873 + Shew_3521 0.34 -4.5 4,208,879 + Shew_3521 0.34 -0.3 4,209,127 + Shew_3521 0.53 -7.3 4,209,411 + Shew_3521 0.76 -5.4 4,209,615 - -7.9 4,209,638 - -6.1 4,209,809 + Shew_3522 0.17 -5.4 4,209,876 + Shew_3522 0.29 -2.0 4,210,277 + -0.0 4,210,477 - Shew_3523 0.13 +0.0 4,210,604 + Shew_3523 0.24 -0.5 4,210,716 + Shew_3523 0.33 -1.3
Or see this region's nucleotide sequence