Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3518 and Shew_3519 overlap by 4 nucleotides Shew_3519 and Shew_3520 overlap by 4 nucleotides Shew_3520 and Shew_3521 overlap by 4 nucleotides
Shew_3518: Shew_3518 - thioesterase superfamily protein (RefSeq), at 4,204,722 to 4,205,156
_3518
Shew_3519: Shew_3519 - outer membrane lipoprotein carrier protein LolA (RefSeq), at 4,205,153 to 4,205,926
_3519
Shew_3520: Shew_3520 - hypothetical protein (RefSeq), at 4,205,923 to 4,208,448
_3520
Shew_3521: Shew_3521 - tryptophan halogenase (RefSeq), at 4,208,445 to 4,209,719
_3521
Position (kb)
4205
4206
4207
4208
4209 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 4204.931 kb on - strand, within Shew_3518 at 4206.357 kb on + strand, within Shew_3520 at 4206.644 kb on + strand, within Shew_3520 at 4206.875 kb on + strand, within Shew_3520 at 4207.091 kb on - strand, within Shew_3520 at 4207.210 kb on - strand, within Shew_3520 at 4207.210 kb on - strand, within Shew_3520 at 4207.274 kb on - strand, within Shew_3520 at 4207.274 kb on - strand, within Shew_3520 at 4207.393 kb on + strand, within Shew_3520 at 4207.549 kb on - strand, within Shew_3520 at 4208.300 kb on - strand at 4208.382 kb on + strand at 4208.392 kb on + strand at 4208.392 kb on + strand at 4208.392 kb on + strand at 4208.400 kb on - strand at 4208.615 kb on + strand, within Shew_3521 at 4208.677 kb on - strand, within Shew_3521 at 4208.847 kb on - strand, within Shew_3521 at 4208.873 kb on + strand, within Shew_3521 at 4208.879 kb on + strand, within Shew_3521 at 4209.127 kb on + strand, within Shew_3521 at 4209.411 kb on + strand, within Shew_3521
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,204,931 - Shew_3518 0.48 -4.1 4,206,357 + Shew_3520 0.17 -3.4 4,206,644 + Shew_3520 0.29 -4.7 4,206,875 + Shew_3520 0.38 -4.4 4,207,091 - Shew_3520 0.46 -5.6 4,207,210 - Shew_3520 0.51 -5.3 4,207,210 - Shew_3520 0.51 -5.9 4,207,274 - Shew_3520 0.53 -6.4 4,207,274 - Shew_3520 0.53 -6.5 4,207,393 + Shew_3520 0.58 -6.1 4,207,549 - Shew_3520 0.64 -6.3 4,208,300 - -7.9 4,208,382 + -4.6 4,208,392 + -5.1 4,208,392 + -8.5 4,208,392 + -5.9 4,208,400 - -6.0 4,208,615 + Shew_3521 0.13 -3.5 4,208,677 - Shew_3521 0.18 -4.2 4,208,847 - Shew_3521 0.32 -8.0 4,208,873 + Shew_3521 0.34 -4.5 4,208,879 + Shew_3521 0.34 -0.3 4,209,127 + Shew_3521 0.53 -7.3 4,209,411 + Shew_3521 0.76 -5.4
Or see this region's nucleotide sequence