Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3263 and Shew_3264 are separated by 33 nucleotides Shew_3264 and Shew_3265 are separated by 525 nucleotides Shew_3265 and dipZ overlap by 4 nucleotides
Shew_3263: Shew_3263 - serine/threonine protein kinase (RefSeq), at 3,900,842 to 3,902,677
_3263
Shew_3264: Shew_3264 - FxsA cytoplasmic membrane protein (RefSeq), at 3,902,711 to 3,903,304
_3264
Shew_3265: Shew_3265 - CutA1 divalent ion tolerance protein (RefSeq), at 3,903,830 to 3,904,153
_3265
Shew_3266: dipZ - thiol:disulfide interchange protein precursor (RefSeq), at 3,904,150 to 3,905,958
dipZ
Position (kb)
3902
3903
3904 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3901.716 kb on + strand, within Shew_3263 at 3901.830 kb on + strand, within Shew_3263 at 3902.084 kb on - strand, within Shew_3263 at 3902.174 kb on - strand, within Shew_3263 at 3902.184 kb on - strand, within Shew_3263 at 3902.184 kb on - strand, within Shew_3263 at 3902.184 kb on - strand, within Shew_3263 at 3902.279 kb on - strand, within Shew_3263 at 3902.919 kb on - strand, within Shew_3264 at 3902.954 kb on - strand, within Shew_3264 at 3903.076 kb on - strand, within Shew_3264 at 3903.221 kb on + strand, within Shew_3264 at 3903.421 kb on - strand at 3903.680 kb on - strand at 3903.788 kb on + strand at 3903.956 kb on + strand, within Shew_3265
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,901,716 + Shew_3263 0.48 +0.6 3,901,830 + Shew_3263 0.54 +1.8 3,902,084 - Shew_3263 0.68 +0.2 3,902,174 - Shew_3263 0.73 +0.0 3,902,184 - Shew_3263 0.73 -1.3 3,902,184 - Shew_3263 0.73 +0.0 3,902,184 - Shew_3263 0.73 +1.0 3,902,279 - Shew_3263 0.78 -0.6 3,902,919 - Shew_3264 0.35 -0.5 3,902,954 - Shew_3264 0.41 -1.5 3,903,076 - Shew_3264 0.61 -0.9 3,903,221 + Shew_3264 0.86 +0.2 3,903,421 - -0.4 3,903,680 - +3.5 3,903,788 + -0.4 3,903,956 + Shew_3265 0.39 -0.1
Or see this region's nucleotide sequence