Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2978 and Shew_2979 are separated by 436 nucleotides Shew_2979 and Shew_2980 are separated by 210 nucleotides Shew_2980 and Shew_2981 are separated by 125 nucleotides
Shew_2978: Shew_2978 - extracellular solute-binding protein (RefSeq), at 3,532,681 to 3,533,427
_2978
Shew_2979: Shew_2979 - putative iron-sulfur cluster binding protein (RefSeq), at 3,533,864 to 3,535,027
_2979
Shew_2980: Shew_2980 - hypothetical protein (RefSeq), at 3,535,238 to 3,535,441
_2980
Shew_2981: Shew_2981 - beta-lactamase domain-containing protein (RefSeq), at 3,535,567 to 3,537,531
_2981
Position (kb)
3533
3534
3535
3536 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3533.218 kb on + strand, within Shew_2978 at 3533.257 kb on - strand, within Shew_2978 at 3533.436 kb on + strand at 3533.441 kb on + strand at 3533.466 kb on + strand at 3533.555 kb on + strand at 3533.623 kb on - strand at 3533.830 kb on + strand at 3533.936 kb on + strand at 3533.944 kb on - strand at 3533.944 kb on - strand at 3534.103 kb on - strand, within Shew_2979 at 3534.478 kb on - strand, within Shew_2979 at 3534.595 kb on + strand, within Shew_2979 at 3534.649 kb on - strand, within Shew_2979 at 3535.012 kb on + strand at 3535.301 kb on - strand, within Shew_2980 at 3535.301 kb on - strand, within Shew_2980 at 3535.329 kb on - strand, within Shew_2980 at 3535.431 kb on - strand at 3535.481 kb on - strand at 3535.552 kb on - strand at 3535.698 kb on + strand at 3535.985 kb on - strand, within Shew_2981
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,533,218 + Shew_2978 0.72 -0.0 3,533,257 - Shew_2978 0.77 -0.2 3,533,436 + -0.0 3,533,441 + -2.8 3,533,466 + +0.3 3,533,555 + +0.1 3,533,623 - +0.2 3,533,830 + +0.4 3,533,936 + -1.0 3,533,944 - -0.6 3,533,944 - -4.6 3,534,103 - Shew_2979 0.21 -1.1 3,534,478 - Shew_2979 0.53 -0.3 3,534,595 + Shew_2979 0.63 -2.1 3,534,649 - Shew_2979 0.67 -0.9 3,535,012 + +0.4 3,535,301 - Shew_2980 0.31 +0.2 3,535,301 - Shew_2980 0.31 -0.2 3,535,329 - Shew_2980 0.45 +0.3 3,535,431 - +0.2 3,535,481 - +0.1 3,535,552 - -0.0 3,535,698 + +0.3 3,535,985 - Shew_2981 0.21 +0.3
Or see this region's nucleotide sequence