Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2937 and Shew_2938 overlap by 20 nucleotides Shew_2938 and Shew_2939 are separated by 135 nucleotides Shew_2939 and Shew_2940 are separated by 134 nucleotides Shew_2940 and Shew_2941 are separated by 89 nucleotides
Shew_2937: Shew_2937 - lytic murein transglycosylase B (RefSeq), at 3,476,776 to 3,477,783
_2937
Shew_2938: Shew_2938 - rare lipoprotein A (RefSeq), at 3,477,764 to 3,478,561
_2938
Shew_2939: Shew_2939 - Serine-type D-Ala-D-Ala carboxypeptidase (RefSeq), at 3,478,697 to 3,479,869
_2939
Shew_2940: Shew_2940 - hypothetical protein (RefSeq), at 3,480,004 to 3,480,270
_2940
Shew_2941: Shew_2941 - lipoate-protein ligase B (RefSeq), at 3,480,360 to 3,481,016
_2941
Position (kb)
3478
3479
3480 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3477.809 kb on - strand at 3478.006 kb on - strand, within Shew_2938 at 3478.006 kb on - strand, within Shew_2938 at 3478.373 kb on + strand, within Shew_2938 at 3478.535 kb on + strand at 3479.131 kb on - strand, within Shew_2939 at 3479.157 kb on - strand, within Shew_2939 at 3479.231 kb on + strand, within Shew_2939 at 3479.457 kb on + strand, within Shew_2939 at 3479.518 kb on - strand, within Shew_2939 at 3479.795 kb on - strand at 3479.795 kb on - strand at 3479.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,477,809 - -4.5 3,478,006 - Shew_2938 0.30 -5.0 3,478,006 - Shew_2938 0.30 -4.7 3,478,373 + Shew_2938 0.76 -1.0 3,478,535 + -0.6 3,479,131 - Shew_2939 0.37 -3.5 3,479,157 - Shew_2939 0.39 -2.9 3,479,231 + Shew_2939 0.46 -3.1 3,479,457 + Shew_2939 0.65 -3.5 3,479,518 - Shew_2939 0.70 -4.2 3,479,795 - -1.2 3,479,795 - -4.9 3,479,795 - -2.8
Or see this region's nucleotide sequence