Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2757 and Shew_2758 are separated by 23 nucleotides Shew_2758 and Shew_2759 are separated by 121 nucleotides Shew_2759 and Shew_2760 are separated by 299 nucleotides
Shew_2757: Shew_2757 - hypothetical protein (RefSeq), at 3,261,443 to 3,261,829
_2757
Shew_2758: Shew_2758 - hypothetical protein (RefSeq), at 3,261,853 to 3,262,335
_2758
Shew_2759: Shew_2759 - peptidase S9 prolyl oligopeptidase (RefSeq), at 3,262,457 to 3,265,270
_2759
Shew_2760: Shew_2760 - isocitrate dehydrogenase (RefSeq), at 3,265,570 to 3,266,577
_2760
Position (kb)
3262
3263
3264
3265
3266 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3261.624 kb on - strand, within Shew_2757 at 3261.675 kb on + strand, within Shew_2757 at 3261.675 kb on + strand, within Shew_2757 at 3261.836 kb on + strand at 3261.852 kb on + strand at 3261.860 kb on - strand at 3261.864 kb on + strand at 3262.491 kb on + strand at 3262.558 kb on + strand at 3262.953 kb on + strand, within Shew_2759 at 3263.063 kb on - strand, within Shew_2759 at 3263.205 kb on - strand, within Shew_2759 at 3263.294 kb on - strand, within Shew_2759 at 3263.358 kb on - strand, within Shew_2759 at 3263.449 kb on - strand, within Shew_2759 at 3263.466 kb on + strand, within Shew_2759 at 3263.544 kb on - strand, within Shew_2759 at 3263.576 kb on - strand, within Shew_2759 at 3263.586 kb on - strand, within Shew_2759 at 3263.679 kb on + strand, within Shew_2759 at 3263.679 kb on + strand, within Shew_2759 at 3263.867 kb on - strand, within Shew_2759 at 3264.028 kb on - strand, within Shew_2759 at 3264.236 kb on - strand, within Shew_2759 at 3264.580 kb on - strand, within Shew_2759 at 3264.700 kb on + strand, within Shew_2759 at 3264.842 kb on + strand, within Shew_2759 at 3265.107 kb on - strand at 3265.108 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,261,624 - Shew_2757 0.47 +0.2 3,261,675 + Shew_2757 0.60 +0.1 3,261,675 + Shew_2757 0.60 -0.1 3,261,836 + -0.0 3,261,852 + -0.2 3,261,860 - +0.6 3,261,864 + +0.1 3,262,491 + +1.7 3,262,558 + +1.3 3,262,953 + Shew_2759 0.18 +1.3 3,263,063 - Shew_2759 0.22 +1.7 3,263,205 - Shew_2759 0.27 +0.9 3,263,294 - Shew_2759 0.30 +0.9 3,263,358 - Shew_2759 0.32 +1.0 3,263,449 - Shew_2759 0.35 +0.7 3,263,466 + Shew_2759 0.36 +1.2 3,263,544 - Shew_2759 0.39 +1.2 3,263,576 - Shew_2759 0.40 +1.1 3,263,586 - Shew_2759 0.40 +1.2 3,263,679 + Shew_2759 0.43 +1.4 3,263,679 + Shew_2759 0.43 +1.0 3,263,867 - Shew_2759 0.50 +1.0 3,264,028 - Shew_2759 0.56 +3.8 3,264,236 - Shew_2759 0.63 +0.7 3,264,580 - Shew_2759 0.75 -0.4 3,264,700 + Shew_2759 0.80 +0.6 3,264,842 + Shew_2759 0.85 +1.0 3,265,107 - +1.2 3,265,108 + +1.4
Or see this region's nucleotide sequence