Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2523 and Shew_2524 are separated by 486 nucleotides Shew_2524 and Shew_2525 are separated by 372 nucleotides
Shew_2523: Shew_2523 - hypothetical protein (RefSeq), at 2,972,045 to 2,974,318
_2523
Shew_2524: Shew_2524 - decaheme cytochrome c (RefSeq), at 2,974,805 to 2,976,982
_2524
Shew_2525: Shew_2525 - decaheme cytochrome c (RefSeq), at 2,977,355 to 2,979,325
_2525
Position (kb)
2974
2975
2976
2977 Strain fitness (log2 ratio)
-2
-1
0
1 at 2973.991 kb on - strand, within Shew_2523 at 2974.241 kb on - strand at 2974.281 kb on + strand at 2974.281 kb on + strand at 2974.371 kb on - strand at 2974.666 kb on + strand at 2974.667 kb on + strand at 2974.967 kb on + strand at 2975.307 kb on - strand, within Shew_2524 at 2975.388 kb on + strand, within Shew_2524 at 2975.799 kb on - strand, within Shew_2524 at 2975.839 kb on - strand, within Shew_2524 at 2976.069 kb on - strand, within Shew_2524 at 2976.283 kb on - strand, within Shew_2524 at 2976.439 kb on - strand, within Shew_2524 at 2976.770 kb on - strand at 2976.777 kb on - strand at 2976.779 kb on + strand at 2976.993 kb on - strand at 2977.072 kb on - strand at 2977.292 kb on - strand at 2977.292 kb on - strand at 2977.400 kb on + strand at 2977.527 kb on - strand at 2977.547 kb on + strand at 2977.708 kb on - strand, within Shew_2525 at 2977.757 kb on - strand, within Shew_2525 at 2977.757 kb on - strand, within Shew_2525
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,973,991 - Shew_2523 0.86 -0.3 2,974,241 - -0.5 2,974,281 + -0.3 2,974,281 + -0.2 2,974,371 - +0.2 2,974,666 + -0.4 2,974,667 + +0.2 2,974,967 + +0.6 2,975,307 - Shew_2524 0.23 -0.9 2,975,388 + Shew_2524 0.27 -2.5 2,975,799 - Shew_2524 0.46 +0.1 2,975,839 - Shew_2524 0.47 -0.2 2,976,069 - Shew_2524 0.58 -2.4 2,976,283 - Shew_2524 0.68 +0.1 2,976,439 - Shew_2524 0.75 -0.3 2,976,770 - +1.1 2,976,777 - +0.1 2,976,779 + +0.3 2,976,993 - -0.3 2,977,072 - -0.0 2,977,292 - +0.2 2,977,292 - +0.3 2,977,400 + -0.1 2,977,527 - -0.0 2,977,547 + +0.3 2,977,708 - Shew_2525 0.18 +0.5 2,977,757 - Shew_2525 0.20 +0.3 2,977,757 - Shew_2525 0.20 -0.1
Or see this region's nucleotide sequence