Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2222 and Shew_2223 are separated by 119 nucleotides Shew_2223 and Shew_2224 are separated by 175 nucleotides Shew_2224 and Shew_2225 are separated by 310 nucleotides
Shew_2222: Shew_2222 - hypothetical protein (RefSeq), at 2,593,467 to 2,593,817
_2222
Shew_2223: Shew_2223 - YceI family protein (RefSeq), at 2,593,937 to 2,594,524
_2223
Shew_2224: Shew_2224 - DNA topoisomerase I (RefSeq), at 2,594,700 to 2,597,396
_2224
Shew_2225: Shew_2225 - succinylarginine dihydrolase (RefSeq), at 2,597,707 to 2,599,044
_2225
Position (kb)
2594
2595
2596
2597
2598 Strain fitness (log2 ratio)
-1
0
1 at 2594.337 kb on - strand, within Shew_2223 at 2594.599 kb on + strand at 2595.120 kb on - strand, within Shew_2224 at 2597.546 kb on - strand at 2597.653 kb on + strand at 2597.653 kb on + strand at 2597.663 kb on + strand at 2597.739 kb on + strand at 2597.994 kb on + strand, within Shew_2225 at 2597.994 kb on + strand, within Shew_2225 at 2597.994 kb on + strand, within Shew_2225 at 2598.002 kb on - strand, within Shew_2225 at 2598.002 kb on - strand, within Shew_2225 at 2598.002 kb on - strand, within Shew_2225 at 2598.002 kb on - strand, within Shew_2225 at 2598.052 kb on - strand, within Shew_2225 at 2598.130 kb on - strand, within Shew_2225 at 2598.312 kb on + strand, within Shew_2225 at 2598.351 kb on + strand, within Shew_2225
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,594,337 - Shew_2223 0.68 -0.8 2,594,599 + +0.4 2,595,120 - Shew_2224 0.16 +0.1 2,597,546 - +0.4 2,597,653 + -0.2 2,597,653 + +0.2 2,597,663 + +0.5 2,597,739 + -0.7 2,597,994 + Shew_2225 0.21 -0.5 2,597,994 + Shew_2225 0.21 +0.0 2,597,994 + Shew_2225 0.21 +0.0 2,598,002 - Shew_2225 0.22 +0.7 2,598,002 - Shew_2225 0.22 +0.5 2,598,002 - Shew_2225 0.22 +0.3 2,598,002 - Shew_2225 0.22 +0.1 2,598,052 - Shew_2225 0.26 -0.1 2,598,130 - Shew_2225 0.32 +1.5 2,598,312 + Shew_2225 0.45 -0.8 2,598,351 + Shew_2225 0.48 +0.0
Or see this region's nucleotide sequence