Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2089 and Shew_2090 are separated by 3 nucleotides Shew_2090 and thiH are separated by 102 nucleotides thiH and Shew_2092 are separated by 4 nucleotides Shew_2092 and Shew_2093 are separated by 1 nucleotides Shew_2093 and Shew_2094 overlap by 8 nucleotides
Shew_2089: Shew_2089 - SrpA-related protein (RefSeq), at 2,416,453 to 2,417,850
_2089
Shew_2090: Shew_2090 - methyl-accepting chemotaxis protein (RefSeq), at 2,417,854 to 2,418,123
_2090
Shew_2091: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,418,226 to 2,419,344
thiH
Shew_2092: Shew_2092 - thiazole biosynthesis family protein (RefSeq), at 2,419,349 to 2,420,122
_2092
Shew_2093: Shew_2093 - thiamine biosynthesis protein ThiS (RefSeq), at 2,420,124 to 2,420,333
_2093
Shew_2094: Shew_2094 - UBA/THIF-type NAD/FAD binding protein (RefSeq), at 2,420,326 to 2,421,174
_2094
Position (kb) 2418
2419
2420 Strain fitness (log2 ratio) -2
-1
0
1 at 2417.296 kb on - strand, within Shew_2089 at 2418.052 kb on + strand, within Shew_2090 at 2419.000 kb on - strand, within thiH at 2419.000 kb on - strand, within thiH at 2419.930 kb on + strand, within Shew_2092
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,417,296 - Shew_2089 0.60 -0.1 2,418,052 + Shew_2090 0.73 +0.1 2,419,000 - thiH Shew_2091 0.69 -1.8 2,419,000 - thiH Shew_2091 0.69 -0.1 2,419,930 + Shew_2092 0.75 -0.3
Or see this region's nucleotide sequence