Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1511 and Shew_1512 are separated by 260 nucleotides Shew_1512 and Shew_1513 overlap by 1 nucleotides Shew_1513 and upp are separated by 211 nucleotides upp and Shew_1515 are separated by 94 nucleotides
Shew_1511: Shew_1511 - UMP phosphatase (RefSeq), at 1,734,073 to 1,734,831
_1511
Shew_1512: Shew_1512 - phosphoribosylglycinamide formyltransferase (RefSeq), at 1,735,092 to 1,735,736
_1512
Shew_1513: Shew_1513 - phosphoribosylaminoimidazole synthetase (RefSeq), at 1,735,736 to 1,736,773
_1513
Shew_1514: upp - uracil phosphoribosyltransferase (RefSeq), at 1,736,985 to 1,737,611
upp
Shew_1515: Shew_1515 - Na+/H+ antiporter NhaC (RefSeq), at 1,737,706 to 1,739,025
_1515
Position (kb) 1735
1736
1737 Strain fitness (log2 ratio) -1
0
1
2
3 at 1734.939 kb on - strand at 1735.002 kb on - strand at 1735.172 kb on + strand, within Shew_1512 at 1735.666 kb on - strand, within Shew_1512 at 1736.251 kb on + strand, within Shew_1513 at 1737.150 kb on + strand, within upp at 1737.570 kb on + strand at 1737.601 kb on + strand at 1737.699 kb on + strand at 1737.759 kb on + strand at 1737.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,734,939 - -0.0 1,735,002 - -1.3 1,735,172 + Shew_1512 0.12 +3.2 1,735,666 - Shew_1512 0.89 +3.0 1,736,251 + Shew_1513 0.50 +2.4 1,737,150 + upp Shew_1514 0.26 -0.9 1,737,570 + -0.9 1,737,601 + -1.0 1,737,699 + -0.3 1,737,759 + +0.0 1,737,767 - +0.0
Or see this region's nucleotide sequence