Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1213 and Shew_1214 are separated by 259 nucleotides Shew_1214 and recA are separated by 330 nucleotides
Shew_1213: Shew_1213 - RpoD family RNA polymerase sigma factor (RefSeq), at 1,406,809 to 1,407,783
_1213
Shew_1214: Shew_1214 - DNA mismatch repair protein (RefSeq), at 1,408,043 to 1,410,610
_1214
Shew_1215: recA - recombinase A (RefSeq), at 1,410,941 to 1,412,002
recA
Position (kb)
1408
1409
1410
1411 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1407.855 kb on + strand at 1407.917 kb on + strand at 1407.917 kb on + strand at 1408.122 kb on - strand at 1408.123 kb on - strand at 1408.132 kb on + strand at 1408.185 kb on - strand at 1408.192 kb on - strand at 1408.202 kb on - strand at 1409.178 kb on - strand, within Shew_1214 at 1409.178 kb on - strand, within Shew_1214 at 1409.220 kb on - strand, within Shew_1214 at 1409.274 kb on - strand, within Shew_1214 at 1409.487 kb on + strand, within Shew_1214 at 1409.654 kb on - strand, within Shew_1214 at 1409.808 kb on - strand, within Shew_1214 at 1410.071 kb on - strand, within Shew_1214 at 1410.236 kb on + strand, within Shew_1214 at 1410.342 kb on + strand, within Shew_1214 at 1410.620 kb on + strand at 1410.783 kb on - strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.864 kb on - strand at 1410.864 kb on - strand at 1410.900 kb on + strand at 1411.185 kb on + strand, within recA at 1411.495 kb on + strand, within recA at 1411.549 kb on - strand, within recA at 1411.575 kb on + strand, within recA
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,407,855 + -0.0 1,407,917 + +0.5 1,407,917 + +0.1 1,408,122 - +0.8 1,408,123 - +0.3 1,408,132 + +1.5 1,408,185 - +1.5 1,408,192 - +0.4 1,408,202 - +0.3 1,409,178 - Shew_1214 0.44 +0.8 1,409,178 - Shew_1214 0.44 +0.7 1,409,220 - Shew_1214 0.46 +0.8 1,409,274 - Shew_1214 0.48 +0.5 1,409,487 + Shew_1214 0.56 +1.1 1,409,654 - Shew_1214 0.63 +0.9 1,409,808 - Shew_1214 0.69 +0.7 1,410,071 - Shew_1214 0.79 +0.9 1,410,236 + Shew_1214 0.85 +0.5 1,410,342 + Shew_1214 0.90 +0.9 1,410,620 + +0.2 1,410,783 - -0.6 1,410,856 + -3.4 1,410,856 + -3.3 1,410,856 + -4.0 1,410,856 + -3.0 1,410,864 - -4.7 1,410,864 - -4.5 1,410,900 + -2.4 1,411,185 + recA Shew_1215 0.23 -3.5 1,411,495 + recA Shew_1215 0.52 -3.1 1,411,549 - recA Shew_1215 0.57 -1.8 1,411,575 + recA Shew_1215 0.60 +0.4
Or see this region's nucleotide sequence