Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1187 and Shew_1188 are separated by 6 nucleotides Shew_1188 and Shew_1189 are separated by 82 nucleotides Shew_1189 and Shew_1190 are separated by 123 nucleotides Shew_1190 and nusB are separated by 14 nucleotides
Shew_1187: Shew_1187 - riboflavin biosynthesis protein RibD (RefSeq), at 1,379,520 to 1,380,680
_1187
Shew_1188: Shew_1188 - riboflavin synthase subunit alpha (RefSeq), at 1,380,687 to 1,381,352
_1188
Shew_1189: Shew_1189 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (RefSeq), at 1,381,435 to 1,382,538
_1189
Shew_1190: Shew_1190 - riboflavin synthase (RefSeq), at 1,382,662 to 1,383,138
_1190
Shew_1191: nusB - transcription antitermination protein NusB (RefSeq), at 1,383,153 to 1,383,557
nusB
Position (kb)
1381
1382
1383 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1380.772 kb on - strand, within Shew_1188 at 1381.246 kb on + strand, within Shew_1188 at 1381.456 kb on - strand at 1381.456 kb on - strand at 1381.697 kb on + strand, within Shew_1189 at 1381.765 kb on + strand, within Shew_1189 at 1381.879 kb on + strand, within Shew_1189 at 1381.905 kb on - strand, within Shew_1189 at 1382.036 kb on - strand, within Shew_1189 at 1382.074 kb on + strand, within Shew_1189 at 1382.449 kb on - strand at 1382.466 kb on + strand at 1382.482 kb on - strand at 1382.483 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,380,772 - Shew_1188 0.13 +3.0 1,381,246 + Shew_1188 0.84 +2.6 1,381,456 - +2.1 1,381,456 - +2.2 1,381,697 + Shew_1189 0.24 +1.8 1,381,765 + Shew_1189 0.30 +1.8 1,381,879 + Shew_1189 0.40 +1.5 1,381,905 - Shew_1189 0.43 +1.9 1,382,036 - Shew_1189 0.54 +2.1 1,382,074 + Shew_1189 0.58 +2.1 1,382,449 - +2.0 1,382,466 + +2.0 1,382,482 - +2.1 1,382,483 - +2.4
Or see this region's nucleotide sequence