Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1157 and Shew_1158 are separated by 14 nucleotides Shew_1158 and Shew_1159 are separated by 271 nucleotides Shew_1159 and Shew_1160 are separated by 184 nucleotides
Shew_1157: Shew_1157 - alkaline phosphatase (RefSeq), at 1,329,935 to 1,331,572
_1157
Shew_1158: Shew_1158 - alkaline phosphatase (RefSeq), at 1,331,587 to 1,333,095
_1158
Shew_1159: Shew_1159 - thioesterase superfamily protein (RefSeq), at 1,333,367 to 1,333,735
_1159
Shew_1160: Shew_1160 - hypothetical protein (RefSeq), at 1,333,920 to 1,334,126
_1160
Position (kb)
1331
1332
1333
1334 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1330.614 kb on - strand, within Shew_1157 at 1330.651 kb on + strand, within Shew_1157 at 1330.659 kb on - strand, within Shew_1157 at 1331.246 kb on + strand, within Shew_1157 at 1331.246 kb on + strand, within Shew_1157 at 1331.251 kb on - strand, within Shew_1157 at 1331.254 kb on - strand, within Shew_1157 at 1331.316 kb on - strand, within Shew_1157 at 1331.661 kb on + strand at 1331.667 kb on + strand at 1331.830 kb on - strand, within Shew_1158 at 1331.837 kb on + strand, within Shew_1158 at 1331.845 kb on - strand, within Shew_1158 at 1332.029 kb on + strand, within Shew_1158 at 1332.056 kb on + strand, within Shew_1158 at 1332.215 kb on + strand, within Shew_1158 at 1332.277 kb on + strand, within Shew_1158 at 1332.322 kb on + strand, within Shew_1158 at 1332.387 kb on - strand, within Shew_1158 at 1332.472 kb on + strand, within Shew_1158 at 1332.605 kb on - strand, within Shew_1158 at 1332.764 kb on + strand, within Shew_1158 at 1332.827 kb on + strand, within Shew_1158 at 1332.879 kb on + strand, within Shew_1158 at 1332.887 kb on - strand, within Shew_1158 at 1332.887 kb on - strand, within Shew_1158 at 1332.887 kb on - strand, within Shew_1158 at 1333.017 kb on - strand at 1333.582 kb on + strand, within Shew_1159 at 1333.585 kb on - strand, within Shew_1159 at 1333.622 kb on - strand, within Shew_1159 at 1333.727 kb on - strand at 1333.793 kb on - strand at 1333.796 kb on + strand at 1333.806 kb on + strand at 1333.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,330,614 - Shew_1157 0.41 -0.4 1,330,651 + Shew_1157 0.44 +0.3 1,330,659 - Shew_1157 0.44 -0.1 1,331,246 + Shew_1157 0.80 -0.5 1,331,246 + Shew_1157 0.80 +1.0 1,331,251 - Shew_1157 0.80 -0.0 1,331,254 - Shew_1157 0.81 -1.5 1,331,316 - Shew_1157 0.84 -0.1 1,331,661 + +0.4 1,331,667 + +0.2 1,331,830 - Shew_1158 0.16 -0.1 1,331,837 + Shew_1158 0.17 -0.2 1,331,845 - Shew_1158 0.17 +0.4 1,332,029 + Shew_1158 0.29 +0.4 1,332,056 + Shew_1158 0.31 -0.2 1,332,215 + Shew_1158 0.42 +0.2 1,332,277 + Shew_1158 0.46 +0.2 1,332,322 + Shew_1158 0.49 -0.1 1,332,387 - Shew_1158 0.53 +1.0 1,332,472 + Shew_1158 0.59 +0.4 1,332,605 - Shew_1158 0.67 +0.1 1,332,764 + Shew_1158 0.78 -0.0 1,332,827 + Shew_1158 0.82 +0.1 1,332,879 + Shew_1158 0.86 -0.0 1,332,887 - Shew_1158 0.86 +0.2 1,332,887 - Shew_1158 0.86 +1.1 1,332,887 - Shew_1158 0.86 +0.2 1,333,017 - +0.2 1,333,582 + Shew_1159 0.58 -0.2 1,333,585 - Shew_1159 0.59 -0.3 1,333,622 - Shew_1159 0.69 +0.7 1,333,727 - +0.4 1,333,793 - +5.0 1,333,796 + +1.4 1,333,806 + -0.1 1,333,814 - +0.2
Or see this region's nucleotide sequence