Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0743 and Shew_0744 are separated by 103 nucleotides Shew_0744 and Shew_0745 are separated by 183 nucleotides Shew_0745 and Shew_0746 are separated by 25 nucleotides
Shew_0743: Shew_0743 - sodium:dicarboxylate symporter (RefSeq), at 844,566 to 845,867
_0743
Shew_0744: Shew_0744 - protein of unknown function DUF306, Meta and HslJ (RefSeq), at 845,971 to 846,384
_0744
Shew_0745: Shew_0745 - hypothetical protein (RefSeq), at 846,568 to 846,807
_0745
Shew_0746: Shew_0746 - diguanylate cyclase (RefSeq), at 846,833 to 847,777
_0746
Position (kb) 845
846
847 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 845.145 kb on - strand, within Shew_0743 at 845.304 kb on + strand, within Shew_0743 at 845.312 kb on - strand, within Shew_0743 at 845.324 kb on + strand, within Shew_0743 at 845.324 kb on + strand, within Shew_0743 at 845.332 kb on - strand, within Shew_0743 at 845.332 kb on - strand, within Shew_0743 at 845.332 kb on - strand, within Shew_0743 at 845.332 kb on - strand, within Shew_0743 at 845.372 kb on + strand, within Shew_0743 at 845.518 kb on - strand, within Shew_0743 at 845.786 kb on + strand at 846.029 kb on + strand, within Shew_0744 at 846.254 kb on + strand, within Shew_0744 at 846.262 kb on - strand, within Shew_0744 at 846.262 kb on - strand, within Shew_0744 at 846.262 kb on - strand, within Shew_0744 at 846.262 kb on - strand, within Shew_0744 at 846.540 kb on + strand at 846.831 kb on + strand at 846.831 kb on + strand at 846.831 kb on + strand at 846.939 kb on - strand, within Shew_0746 at 846.949 kb on - strand, within Shew_0746 at 847.065 kb on - strand, within Shew_0746
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 845,145 - Shew_0743 0.44 +0.2 845,304 + Shew_0743 0.57 +0.3 845,312 - Shew_0743 0.57 +0.0 845,324 + Shew_0743 0.58 -0.1 845,324 + Shew_0743 0.58 -0.2 845,332 - Shew_0743 0.59 +2.2 845,332 - Shew_0743 0.59 +0.1 845,332 - Shew_0743 0.59 +0.8 845,332 - Shew_0743 0.59 +0.3 845,372 + Shew_0743 0.62 -0.1 845,518 - Shew_0743 0.73 +0.4 845,786 + +0.0 846,029 + Shew_0744 0.14 -0.5 846,254 + Shew_0744 0.68 -1.3 846,262 - Shew_0744 0.70 -2.8 846,262 - Shew_0744 0.70 -1.7 846,262 - Shew_0744 0.70 -3.7 846,262 - Shew_0744 0.70 -1.8 846,540 + +0.7 846,831 + +0.1 846,831 + +0.3 846,831 + -0.0 846,939 - Shew_0746 0.11 -0.2 846,949 - Shew_0746 0.12 +0.0 847,065 - Shew_0746 0.25 +0.3
Or see this region's nucleotide sequence