Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0672 and Shew_0673 are separated by 8 nucleotides Shew_0673 and Shew_0674 are separated by 98 nucleotides Shew_0674 and Shew_0675 are separated by 202 nucleotides
Shew_0672: Shew_0672 - uroporphyrin-III C-methyltransferase (RefSeq), at 765,289 to 766,110
_0672
Shew_0673: Shew_0673 - hypothetical protein (RefSeq), at 766,119 to 766,955
_0673
Shew_0674: Shew_0674 - major facilitator transporter (RefSeq), at 767,054 to 768,220
_0674
Shew_0675: Shew_0675 - hypothetical protein (RefSeq), at 768,423 to 769,868
_0675
Position (kb) 767
768
769 Strain fitness (log2 ratio) -1
0
1
2 at 766.160 kb on - strand at 766.292 kb on + strand, within Shew_0673 at 766.365 kb on + strand, within Shew_0673 at 766.526 kb on - strand, within Shew_0673 at 766.618 kb on + strand, within Shew_0673 at 766.724 kb on - strand, within Shew_0673 at 766.935 kb on - strand at 767.040 kb on - strand at 767.046 kb on + strand at 767.143 kb on - strand at 767.187 kb on + strand, within Shew_0674 at 767.360 kb on - strand, within Shew_0674 at 767.645 kb on + strand, within Shew_0674 at 767.780 kb on + strand, within Shew_0674 at 767.886 kb on - strand, within Shew_0674 at 767.912 kb on + strand, within Shew_0674 at 768.450 kb on - strand at 768.701 kb on - strand, within Shew_0675 at 768.783 kb on + strand, within Shew_0675 at 769.087 kb on - strand, within Shew_0675
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 766,160 - -0.0 766,292 + Shew_0673 0.21 +0.3 766,365 + Shew_0673 0.29 -0.3 766,526 - Shew_0673 0.49 -0.2 766,618 + Shew_0673 0.60 +0.0 766,724 - Shew_0673 0.72 +0.2 766,935 - -0.2 767,040 - -0.3 767,046 + -0.4 767,143 - +0.1 767,187 + Shew_0674 0.11 +0.6 767,360 - Shew_0674 0.26 +0.1 767,645 + Shew_0674 0.51 -0.3 767,780 + Shew_0674 0.62 +0.7 767,886 - Shew_0674 0.71 +1.8 767,912 + Shew_0674 0.74 +0.7 768,450 - +0.8 768,701 - Shew_0675 0.19 -0.3 768,783 + Shew_0675 0.25 +0.3 769,087 - Shew_0675 0.46 -1.4
Or see this region's nucleotide sequence