Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0563 and Shew_0564 are separated by 9 nucleotides Shew_0564 and Shew_0565 overlap by 8 nucleotides
Shew_0563: Shew_0563 - N-acetylmuramoyl-L-alanine amidase (RefSeq), at 637,936 to 639,255
_0563
Shew_0564: Shew_0564 - DNA mismatch repair protein MutL (RefSeq), at 639,265 to 641,160
_0564
Shew_0565: Shew_0565 - tRNA delta(2)-isopentenylpyrophosphate transferase (RefSeq), at 641,153 to 642,079
_0565
Position (kb) 639
640
641
642 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 638.948 kb on + strand, within Shew_0563 at 639.209 kb on - strand at 639.355 kb on + strand at 639.363 kb on - strand at 639.363 kb on - strand at 639.478 kb on - strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.613 kb on - strand, within Shew_0564 at 639.638 kb on - strand, within Shew_0564 at 639.652 kb on - strand, within Shew_0564 at 639.962 kb on + strand, within Shew_0564 at 640.178 kb on + strand, within Shew_0564 at 640.186 kb on - strand, within Shew_0564 at 640.225 kb on - strand, within Shew_0564 at 640.519 kb on + strand, within Shew_0564 at 640.586 kb on - strand, within Shew_0564 at 640.643 kb on + strand, within Shew_0564 at 640.699 kb on + strand, within Shew_0564 at 641.242 kb on + strand at 641.590 kb on + strand, within Shew_0565 at 641.717 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 638,948 + Shew_0563 0.77 -3.7 639,209 - +1.4 639,355 + +0.8 639,363 - +1.0 639,363 - +0.6 639,478 - Shew_0564 0.11 +1.6 639,494 + Shew_0564 0.12 +1.9 639,494 + Shew_0564 0.12 +1.0 639,494 + Shew_0564 0.12 +1.1 639,613 - Shew_0564 0.18 +1.0 639,638 - Shew_0564 0.20 +0.5 639,652 - Shew_0564 0.20 +1.6 639,962 + Shew_0564 0.37 +1.4 640,178 + Shew_0564 0.48 +0.9 640,186 - Shew_0564 0.49 -1.5 640,225 - Shew_0564 0.51 +0.9 640,519 + Shew_0564 0.66 +1.1 640,586 - Shew_0564 0.70 +0.8 640,643 + Shew_0564 0.73 +0.6 640,699 + Shew_0564 0.76 +1.2 641,242 + -2.8 641,590 + Shew_0565 0.47 -2.3 641,717 + Shew_0565 0.61 -3.4 641,768 + Shew_0565 0.66 -2.7 641,768 + Shew_0565 0.66 -3.6 641,987 + -2.6
Or see this region's nucleotide sequence