Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0526 and Shew_0527 are separated by 42 nucleotides Shew_0527 and Shew_0528 are separated by 94 nucleotides Shew_0528 and Shew_0529 are separated by 79 nucleotides
Shew_0526: Shew_0526 - hypothetical protein (RefSeq), at 596,035 to 596,406
_0526
Shew_0527: Shew_0527 - Sel1 domain-containing protein (RefSeq), at 596,449 to 597,303
_0527
Shew_0528: Shew_0528 - Sel1 domain-containing protein (RefSeq), at 597,398 to 598,222
_0528
Shew_0529: Shew_0529 - Sel1 domain-containing protein (RefSeq), at 598,302 to 599,129
_0529
Position (kb) 597
598
599 Strain fitness (log2 ratio) -2
-1
0
1 at 596.464 kb on + strand at 596.543 kb on + strand, within Shew_0527 at 596.690 kb on - strand, within Shew_0527 at 596.791 kb on - strand, within Shew_0527 at 596.801 kb on - strand, within Shew_0527 at 596.891 kb on - strand, within Shew_0527 at 596.917 kb on - strand, within Shew_0527 at 597.029 kb on + strand, within Shew_0527 at 597.029 kb on + strand, within Shew_0527 at 597.226 kb on - strand at 597.236 kb on + strand at 597.444 kb on - strand at 597.450 kb on - strand at 597.887 kb on - strand, within Shew_0528 at 597.945 kb on + strand, within Shew_0528 at 597.953 kb on - strand, within Shew_0528 at 597.992 kb on - strand, within Shew_0528 at 598.180 kb on + strand at 598.698 kb on - strand, within Shew_0529 at 599.062 kb on + strand at 599.219 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 596,464 + -0.0 596,543 + Shew_0527 0.11 -0.1 596,690 - Shew_0527 0.28 -0.1 596,791 - Shew_0527 0.40 +0.0 596,801 - Shew_0527 0.41 -1.6 596,891 - Shew_0527 0.52 -0.5 596,917 - Shew_0527 0.55 -0.4 597,029 + Shew_0527 0.68 +0.3 597,029 + Shew_0527 0.68 +0.2 597,226 - -0.2 597,236 + -1.6 597,444 - -0.0 597,450 - -0.4 597,887 - Shew_0528 0.59 -0.7 597,945 + Shew_0528 0.66 -0.0 597,953 - Shew_0528 0.67 -0.3 597,992 - Shew_0528 0.72 -0.8 598,180 + +0.2 598,698 - Shew_0529 0.48 -0.2 599,062 + -2.2 599,219 + +0.9
Or see this region's nucleotide sequence