Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0496 and Shew_0497 are separated by 892 nucleotides Shew_0497 and Shew_0498 are separated by 5 nucleotides Shew_0498 and Shew_0499 are separated by 145 nucleotides
Shew_0496: Shew_0496 - amino acid carrier protein (RefSeq), at 553,040 to 554,413
_0496
Shew_0497: Shew_0497 - putative sulfate transporter YchM (RefSeq), at 555,306 to 557,003
_0497
Shew_0498: Shew_0498 - flavoprotein oxygenase (RefSeq), at 557,009 to 557,638
_0498
Shew_0499: Shew_0499 - Xaa-Pro aminopeptidase family enzyme (RefSeq), at 557,784 to 559,136
_0499
Position (kb) 555
556
557
558 Strain fitness (log2 ratio) -2
-1
0
1 at 554.450 kb on + strand at 554.539 kb on - strand at 554.573 kb on + strand at 554.670 kb on + strand at 554.737 kb on + strand at 555.009 kb on + strand at 555.036 kb on + strand at 555.049 kb on - strand at 555.049 kb on - strand at 555.202 kb on - strand at 555.569 kb on - strand, within Shew_0497 at 555.742 kb on + strand, within Shew_0497 at 555.787 kb on + strand, within Shew_0497 at 555.793 kb on - strand, within Shew_0497 at 555.809 kb on - strand, within Shew_0497 at 555.841 kb on + strand, within Shew_0497 at 556.026 kb on - strand, within Shew_0497 at 556.082 kb on + strand, within Shew_0497 at 556.218 kb on - strand, within Shew_0497 at 556.239 kb on - strand, within Shew_0497 at 556.241 kb on + strand, within Shew_0497 at 556.262 kb on + strand, within Shew_0497 at 556.332 kb on + strand, within Shew_0497 at 556.609 kb on + strand, within Shew_0497 at 556.609 kb on + strand, within Shew_0497 at 556.617 kb on - strand, within Shew_0497 at 556.617 kb on - strand, within Shew_0497 at 556.617 kb on - strand, within Shew_0497 at 556.617 kb on - strand, within Shew_0497 at 556.617 kb on - strand, within Shew_0497 at 557.090 kb on - strand, within Shew_0498 at 557.102 kb on - strand, within Shew_0498 at 557.170 kb on + strand, within Shew_0498 at 557.178 kb on - strand, within Shew_0498 at 557.453 kb on + strand, within Shew_0498 at 557.885 kb on - strand at 558.001 kb on + strand, within Shew_0499
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 554,450 + -1.0 554,539 - +0.0 554,573 + +0.0 554,670 + +1.7 554,737 + -0.2 555,009 + +0.4 555,036 + +0.1 555,049 - -0.3 555,049 - +0.7 555,202 - -0.5 555,569 - Shew_0497 0.15 -0.1 555,742 + Shew_0497 0.26 +1.2 555,787 + Shew_0497 0.28 -0.1 555,793 - Shew_0497 0.29 -0.3 555,809 - Shew_0497 0.30 +0.0 555,841 + Shew_0497 0.32 -0.9 556,026 - Shew_0497 0.42 -0.5 556,082 + Shew_0497 0.46 -0.2 556,218 - Shew_0497 0.54 +1.6 556,239 - Shew_0497 0.55 +0.2 556,241 + Shew_0497 0.55 +0.3 556,262 + Shew_0497 0.56 -0.4 556,332 + Shew_0497 0.60 -0.3 556,609 + Shew_0497 0.77 -0.3 556,609 + Shew_0497 0.77 -0.3 556,617 - Shew_0497 0.77 -0.1 556,617 - Shew_0497 0.77 +0.4 556,617 - Shew_0497 0.77 -0.6 556,617 - Shew_0497 0.77 +0.5 556,617 - Shew_0497 0.77 +0.3 557,090 - Shew_0498 0.13 +0.2 557,102 - Shew_0498 0.15 -0.2 557,170 + Shew_0498 0.26 -0.1 557,178 - Shew_0498 0.27 -1.5 557,453 + Shew_0498 0.70 -1.9 557,885 - +0.1 558,001 + Shew_0499 0.16 +0.1
Or see this region's nucleotide sequence