Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0449 and Shew_0450 are separated by 235 nucleotides Shew_0450 and Shew_0451 are separated by 24 nucleotides Shew_0451 and Shew_0452 are separated by 77 nucleotides
Shew_0449: Shew_0449 - ABC-3 protein (RefSeq), at 500,159 to 500,950
_0449
Shew_0450: Shew_0450 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (RefSeq), at 501,186 to 501,920
_0450
Shew_0451: Shew_0451 - hypothetical protein (RefSeq), at 501,945 to 502,352
_0451
Shew_0452: Shew_0452 - diguanylate cyclase (RefSeq), at 502,430 to 503,401
_0452
Position (kb) 501
502 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 500.331 kb on - strand, within Shew_0449 at 500.575 kb on - strand, within Shew_0449 at 500.708 kb on - strand, within Shew_0449 at 500.902 kb on + strand at 501.451 kb on + strand, within Shew_0450 at 501.459 kb on - strand, within Shew_0450 at 501.462 kb on + strand, within Shew_0450 at 502.042 kb on + strand, within Shew_0451 at 502.225 kb on + strand, within Shew_0451 at 502.430 kb on + strand at 502.568 kb on + strand, within Shew_0452 at 502.916 kb on + strand, within Shew_0452
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 500,331 - Shew_0449 0.22 -0.4 500,575 - Shew_0449 0.53 -0.0 500,708 - Shew_0449 0.69 -0.1 500,902 + -0.0 501,451 + Shew_0450 0.36 -2.0 501,459 - Shew_0450 0.37 -3.3 501,462 + Shew_0450 0.38 -1.3 502,042 + Shew_0451 0.24 +0.4 502,225 + Shew_0451 0.69 +0.5 502,430 + -0.1 502,568 + Shew_0452 0.14 +0.6 502,916 + Shew_0452 0.50 +0.1
Or see this region's nucleotide sequence