Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0399 and Shew_0400 overlap by 1 nucleotides Shew_0400 and Shew_0401 overlap by 11 nucleotides Shew_0401 and Shew_0402 overlap by 8 nucleotides
Shew_0399: Shew_0399 - methylation site containing protein (RefSeq), at 448,358 to 448,909
_0399
Shew_0400: Shew_0400 - methylation site containing protein (RefSeq), at 448,909 to 449,706
_0400
Shew_0401: Shew_0401 - MSHA biogenesis protein MshP (RefSeq), at 449,696 to 450,133
_0401
Shew_0402: Shew_0402 - MSHA biogenesis protein MshQ (RefSeq), at 450,126 to 452,828
_0402
Position (kb) 449
450
451 Strain fitness (log2 ratio) -2
-1
0
1
2 at 448.727 kb on - strand, within Shew_0399 at 448.743 kb on - strand, within Shew_0399 at 448.881 kb on + strand at 448.903 kb on + strand at 449.041 kb on + strand, within Shew_0400 at 449.097 kb on + strand, within Shew_0400 at 449.105 kb on - strand, within Shew_0400 at 449.105 kb on - strand, within Shew_0400 at 449.125 kb on - strand, within Shew_0400 at 449.252 kb on + strand, within Shew_0400 at 449.313 kb on - strand, within Shew_0400 at 449.363 kb on - strand, within Shew_0400 at 449.418 kb on - strand, within Shew_0400 at 449.418 kb on - strand, within Shew_0400 at 449.642 kb on + strand at 449.989 kb on - strand, within Shew_0401 at 450.014 kb on + strand, within Shew_0401 at 450.170 kb on + strand at 450.321 kb on - strand at 451.130 kb on + strand, within Shew_0402
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 448,727 - Shew_0399 0.67 +1.5 448,743 - Shew_0399 0.70 +0.7 448,881 + +0.6 448,903 + -0.8 449,041 + Shew_0400 0.17 +1.2 449,097 + Shew_0400 0.24 +0.8 449,105 - Shew_0400 0.25 +1.0 449,105 - Shew_0400 0.25 +0.0 449,125 - Shew_0400 0.27 +0.4 449,252 + Shew_0400 0.43 +0.6 449,313 - Shew_0400 0.51 +2.3 449,363 - Shew_0400 0.57 +0.8 449,418 - Shew_0400 0.64 +0.4 449,418 - Shew_0400 0.64 +0.8 449,642 + +0.4 449,989 - Shew_0401 0.67 +1.3 450,014 + Shew_0401 0.73 +0.8 450,170 + +0.5 450,321 - -2.1 451,130 + Shew_0402 0.37 +0.6
Or see this region's nucleotide sequence