Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rho and Shew_0338 are separated by 230 nucleotides Shew_0338 and Shew_0339 are separated by 171 nucleotides Shew_0339 and Shew_0340 overlap by 1 nucleotides
Shew_0337: rho - transcription termination factor Rho (RefSeq), at 382,589 to 383,854
rho
Shew_0338: Shew_0338 - thioredoxin (RefSeq), at 384,085 to 384,411
_0338
Shew_0339: Shew_0339 - ATP-dependent RNA helicase RhlB (RefSeq), at 384,583 to 385,890
_0339
Shew_0340: Shew_0340 - Ppx/GppA phosphatase (RefSeq), at 385,890 to 386,822
_0340
Position (kb) 384
385
386 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 383.871 kb on - strand at 383.871 kb on - strand at 384.085 kb on + strand at 384.091 kb on - strand at 384.673 kb on - strand at 384.690 kb on + strand at 384.818 kb on + strand, within Shew_0339 at 384.847 kb on - strand, within Shew_0339 at 384.917 kb on + strand, within Shew_0339 at 384.934 kb on + strand, within Shew_0339 at 384.948 kb on + strand, within Shew_0339 at 385.403 kb on - strand, within Shew_0339 at 385.405 kb on + strand, within Shew_0339 at 385.453 kb on - strand, within Shew_0339 at 385.555 kb on - strand, within Shew_0339 at 385.739 kb on + strand, within Shew_0339 at 386.132 kb on + strand, within Shew_0340 at 386.207 kb on - strand, within Shew_0340 at 386.559 kb on + strand, within Shew_0340
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 383,871 - -2.2 383,871 - -1.7 384,085 + -0.1 384,091 - -0.8 384,673 - -3.5 384,690 + -3.1 384,818 + Shew_0339 0.18 -2.5 384,847 - Shew_0339 0.20 -0.6 384,917 + Shew_0339 0.26 -1.7 384,934 + Shew_0339 0.27 -2.3 384,948 + Shew_0339 0.28 -0.3 385,403 - Shew_0339 0.63 -1.9 385,405 + Shew_0339 0.63 -3.5 385,453 - Shew_0339 0.67 -3.2 385,555 - Shew_0339 0.74 -3.0 385,739 + Shew_0339 0.88 -1.7 386,132 + Shew_0340 0.26 -0.3 386,207 - Shew_0340 0.34 -0.2 386,559 + Shew_0340 0.72 -0.5
Or see this region's nucleotide sequence