Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0325 and Shew_0326 overlap by 8 nucleotides Shew_0326 and Shew_0327 are separated by 101 nucleotides Shew_0327 and Shew_0328 are separated by 112 nucleotides
Shew_0325: Shew_0325 - tyrosine recombinase XerC (RefSeq), at 371,031 to 371,924
_0325
Shew_0326: Shew_0326 - HAD family hydrolase (RefSeq), at 371,917 to 372,639
_0326
Shew_0327: Shew_0327 - HupE/UreJ protein (RefSeq), at 372,741 to 373,316
_0327
Shew_0328: Shew_0328 - hypothetical protein (RefSeq), at 373,429 to 374,145
_0328
Position (kb) 372
373
374 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 371.742 kb on - strand, within Shew_0325 at 371.805 kb on + strand, within Shew_0325 at 371.837 kb on + strand at 371.837 kb on + strand at 371.845 kb on - strand at 371.874 kb on + strand at 372.400 kb on + strand, within Shew_0326 at 372.413 kb on + strand, within Shew_0326 at 372.474 kb on - strand, within Shew_0326 at 372.750 kb on + strand at 373.140 kb on + strand, within Shew_0327 at 373.145 kb on + strand, within Shew_0327 at 373.148 kb on - strand, within Shew_0327 at 373.554 kb on + strand, within Shew_0328 at 373.647 kb on - strand, within Shew_0328 at 374.191 kb on + strand at 374.191 kb on + strand at 374.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 371,742 - Shew_0325 0.80 -0.6 371,805 + Shew_0325 0.87 -2.0 371,837 + -1.8 371,837 + -5.4 371,845 - -0.3 371,874 + -3.0 372,400 + Shew_0326 0.67 +3.1 372,413 + Shew_0326 0.69 +3.0 372,474 - Shew_0326 0.77 +3.0 372,750 + -0.4 373,140 + Shew_0327 0.69 -1.3 373,145 + Shew_0327 0.70 -0.2 373,148 - Shew_0327 0.71 -2.1 373,554 + Shew_0328 0.17 +0.4 373,647 - Shew_0328 0.30 -0.3 374,191 + -0.2 374,191 + +1.2 374,199 - +0.1
Or see this region's nucleotide sequence