Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0264 and Shew_0265 are separated by 129 nucleotides Shew_0265 and Shew_0266 are separated by 337 nucleotides Shew_0266 and Shew_0267 are separated by 69 nucleotides
Shew_0264: Shew_0264 - serine/threonine protein kinase (RefSeq), at 282,793 to 283,794
_0264
Shew_0265: Shew_0265 - DSBA oxidoreductase (RefSeq), at 283,924 to 284,535
_0265
Shew_0266: Shew_0266 - hypothetical protein (RefSeq), at 284,873 to 285,085
_0266
Shew_0267: Shew_0267 - hypothetical protein (RefSeq), at 285,155 to 285,361
_0267
Position (kb) 283
284
285 Strain fitness (log2 ratio) -2
-1
0
1 at 283.020 kb on + strand, within Shew_0264 at 283.087 kb on + strand, within Shew_0264 at 283.157 kb on + strand, within Shew_0264 at 283.157 kb on + strand, within Shew_0264 at 283.165 kb on - strand, within Shew_0264 at 283.347 kb on - strand, within Shew_0264 at 283.356 kb on + strand, within Shew_0264 at 284.091 kb on - strand, within Shew_0265 at 284.211 kb on - strand, within Shew_0265 at 284.366 kb on + strand, within Shew_0265 at 284.486 kb on - strand at 285.085 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 283,020 + Shew_0264 0.23 -0.4 283,087 + Shew_0264 0.29 -0.1 283,157 + Shew_0264 0.36 +0.2 283,157 + Shew_0264 0.36 +0.7 283,165 - Shew_0264 0.37 -0.2 283,347 - Shew_0264 0.55 +0.2 283,356 + Shew_0264 0.56 -0.1 284,091 - Shew_0265 0.27 -2.1 284,211 - Shew_0265 0.47 -0.4 284,366 + Shew_0265 0.72 -0.7 284,486 - -0.5 285,085 + -0.4
Or see this region's nucleotide sequence