Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0223 and Shew_0224 are separated by 21 nucleotides Shew_0224 and Shew_0225 overlap by 4 nucleotides Shew_0225 and Shew_0226 are separated by 68 nucleotides
Shew_0223: Shew_0223 - hypothetical protein (RefSeq), at 237,090 to 237,959
_0223
Shew_0224: Shew_0224 - transport-associated (RefSeq), at 237,981 to 238,550
_0224
Shew_0225: Shew_0225 - phosphoheptose isomerase (RefSeq), at 238,547 to 239,140
_0225
Shew_0226: Shew_0226 - hypothetical protein (RefSeq), at 239,209 to 239,553
_0226
Shew_0227: Shew_0227 - LppC family lipoprotein (RefSeq), at 239,550 to 241,454
_0227
Position (kb) 237
238
239 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 237.118 kb on + strand at 237.322 kb on + strand, within Shew_0223 at 237.388 kb on - strand, within Shew_0223 at 237.693 kb on + strand, within Shew_0223 at 237.693 kb on + strand, within Shew_0223 at 237.705 kb on + strand, within Shew_0223 at 237.711 kb on - strand, within Shew_0223 at 237.768 kb on + strand, within Shew_0223 at 238.094 kb on - strand, within Shew_0224 at 238.167 kb on - strand, within Shew_0224 at 238.292 kb on + strand, within Shew_0224 at 238.300 kb on - strand, within Shew_0224 at 239.042 kb on + strand, within Shew_0225 at 239.254 kb on - strand, within Shew_0226 at 239.495 kb on - strand, within Shew_0226
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 237,118 + +0.7 237,322 + Shew_0223 0.27 -0.2 237,388 - Shew_0223 0.34 +0.6 237,693 + Shew_0223 0.69 -0.4 237,693 + Shew_0223 0.69 +0.3 237,705 + Shew_0223 0.71 +0.2 237,711 - Shew_0223 0.71 +0.2 237,768 + Shew_0223 0.78 +0.2 238,094 - Shew_0224 0.20 -4.6 238,167 - Shew_0224 0.33 -3.9 238,292 + Shew_0224 0.55 -2.3 238,300 - Shew_0224 0.56 -4.4 239,042 + Shew_0225 0.83 -3.2 239,254 - Shew_0226 0.13 -0.7 239,495 - Shew_0226 0.83 -0.1
Or see this region's nucleotide sequence